vector carrying ace2 coding sequences (Addgene inc)
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Vector Carrying Ace2 Coding Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+coding+sequence/ACE2+g1+%2B+Cas9+(Plasmid+%23153011)/pm40133950-166-13-23
Average 91 stars, based on 1 article reviews
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Sequencing:Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization. Article Snippet: .. To remove Article Title: Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila Article Snippet: Gal4 5’ and 3’ homology arms were PCR-amplified using pDEST-APIGH (Addgene # 112804) as the template. .. Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters Article Snippet: The pSBP2APuro plasmid was digested with PvuII and XhoI, blunted with New England Biolabs Quick Blunting kit (cat# E1201S), and re-ligated to remove excess unnecessary DNA sequence. .. The Article Title: ANGPLT3 Impacts Not Only Proteinuria But Also Hyperlipidemia in Nephrotic Syndrome Article Snippet: .. Production of Cas9 mRNA and sgRNA T7 promoter was added to Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization Article Snippet: .. To remove Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Plasmid Preparation:Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization. Article Snippet: .. To remove Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters Article Snippet: The pSBP2APuro plasmid was digested with PvuII and XhoI, blunted with New England Biolabs Quick Blunting kit (cat# E1201S), and re-ligated to remove excess unnecessary DNA sequence. .. The Article Title: ANGPLT3 Impacts Not Only Proteinuria But Also Hyperlipidemia in Nephrotic Syndrome Article Snippet: .. Production of Cas9 mRNA and sgRNA T7 promoter was added to Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization Article Snippet: .. To remove Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Polymerase Chain Reaction:Article Title: Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila Article Snippet: Gal4 5’ and 3’ homology arms were PCR-amplified using pDEST-APIGH (Addgene # 112804) as the template. .. Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Article Title: ANGPLT3 Impacts Not Only Proteinuria But Also Hyperlipidemia in Nephrotic Syndrome Article Snippet: .. Production of Cas9 mRNA and sgRNA T7 promoter was added to Construct:Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Virus:Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Clone Assay:Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the other:Article Title: Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides Article Snippet: Production of Cas9 mRNA and sn1-casRNA/sn2-casRNA A T7 promoter is added to Cas9 coding region optimized for mammalian Over Expression:Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Expressing:Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a |
