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GEM reduced cancer cell survival in multiple cancer cell lines in a concentration-dependent manner. ( A – H ) Dose–response curve for breast (MCF7, MDA MB 231), lung (A549, H292), pancreatic (Panc-1, MIA PaCa-2, <t>Capan-1)</t> and stomach (AGS) cancer cell lines after treatment with Gemcitabine (GEM) for 48 h. ( I ) Calculated GEM IC50 values for the various cancer cell lines. Data points are represented as means ± SEMs; n = 3. a IC50 was calculated using non-linear regression (R) from cell survival dose–response curves. b ND: Not determined due to the minimal toxicity of GEM to MDA MB 231. c ND: Not determined due to GEM’s moderate cytotoxicity at 10,000 µM.
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GEM reduced cancer cell survival in multiple cancer cell lines in a concentration-dependent manner. ( A – H ) Dose–response curve for breast (MCF7, MDA MB 231), lung (A549, H292), pancreatic (Panc-1, MIA PaCa-2, Capan-1) and stomach (AGS) cancer cell lines after treatment with Gemcitabine (GEM) for 48 h. ( I ) Calculated GEM IC50 values for the various cancer cell lines. Data points are represented as means ± SEMs; n = 3. a IC50 was calculated using non-linear regression (R) from cell survival dose–response curves. b ND: Not determined due to the minimal toxicity of GEM to MDA MB 231. c ND: Not determined due to GEM’s moderate cytotoxicity at 10,000 µM.

Journal: Molecules

Article Title: Identification of Putative Equilibrative Nucleoside Transporter Inhibitors Through Dual-Pharmacophore Virtual Screening and Validation in a Gemcitabine-Based Cell Assay

doi: 10.3390/molecules31081293

Figure Lengend Snippet: GEM reduced cancer cell survival in multiple cancer cell lines in a concentration-dependent manner. ( A – H ) Dose–response curve for breast (MCF7, MDA MB 231), lung (A549, H292), pancreatic (Panc-1, MIA PaCa-2, Capan-1) and stomach (AGS) cancer cell lines after treatment with Gemcitabine (GEM) for 48 h. ( I ) Calculated GEM IC50 values for the various cancer cell lines. Data points are represented as means ± SEMs; n = 3. a IC50 was calculated using non-linear regression (R) from cell survival dose–response curves. b ND: Not determined due to the minimal toxicity of GEM to MDA MB 231. c ND: Not determined due to GEM’s moderate cytotoxicity at 10,000 µM.

Article Snippet: The gastric adenocarcinoma cell line AGS (ATCC CRL1739) was a gift from Sharjah University, while the pancreatic cell lines Capan-1 (ATCC CLS-300143), PANC-1 (ATCC CRL-1469), and MIA-PaCa-2 (ATCC CRL-1420) were gifts from the Gulf Medical School (UAE).

Techniques: Concentration Assay

NBTI blocked GEM-induced cytotoxicity in a subset of cancer cell lines. 4-nitrobenzylthioinosine (NBTI)’s effect on GEM-induced cytotoxicity in five cancer cell lines: ( A ) MCF7, ( B ) AGS, ( C ) A549, ( D ) Capan-1 and ( E ) H292. ( F ) Increasing NBTI concentrations shifted the GEM dose–response curve to the right, increasing GEM IC50 values in H292 cells ( G ). a IC50 values were calculated using non-linear regression. Data represent means ± SEMs; n = 3–5; Ϯ p < 0.001 GEM vs. control; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. GEM-treated cells.

Journal: Molecules

Article Title: Identification of Putative Equilibrative Nucleoside Transporter Inhibitors Through Dual-Pharmacophore Virtual Screening and Validation in a Gemcitabine-Based Cell Assay

doi: 10.3390/molecules31081293

Figure Lengend Snippet: NBTI blocked GEM-induced cytotoxicity in a subset of cancer cell lines. 4-nitrobenzylthioinosine (NBTI)’s effect on GEM-induced cytotoxicity in five cancer cell lines: ( A ) MCF7, ( B ) AGS, ( C ) A549, ( D ) Capan-1 and ( E ) H292. ( F ) Increasing NBTI concentrations shifted the GEM dose–response curve to the right, increasing GEM IC50 values in H292 cells ( G ). a IC50 values were calculated using non-linear regression. Data represent means ± SEMs; n = 3–5; Ϯ p < 0.001 GEM vs. control; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. GEM-treated cells.

Article Snippet: The gastric adenocarcinoma cell line AGS (ATCC CRL1739) was a gift from Sharjah University, while the pancreatic cell lines Capan-1 (ATCC CLS-300143), PANC-1 (ATCC CRL-1469), and MIA-PaCa-2 (ATCC CRL-1420) were gifts from the Gulf Medical School (UAE).

Techniques: Control