Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure (mg/kg body weight/day) to canthaxanthin calculated based on existing MRLs of 15 mg canthaxanthin/kg liver (wet tissue) and 2.5 mg canthaxanthin/kg skin/fat (wet tissue).

Article Snippet: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.) .

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure per population class, country and survey of consumers (mg/kg bw per day) to canthaxanthin based on existing MRLs for Carophyll Red 10% (2.5 mg/kg skin/fat, 15 mg/kg liver).

Article Snippet: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.) .

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure to canthaxanthin (mg/kg body weight/day) calculated based on existing MRLs in poultry and salmonids.

Article Snippet: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.) .

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure per population class, country and survey of consumers (mg/kg bw per day) to canthaxanthin calculated based on existing MRLs in poultry (2.5 mg/kg skin/fat, 15 mg/kg liver, 8.1 mg/kg whole egg) and existing MRL in trout/salmon (10 mg/kg muscle).

Article Snippet: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.) .

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure (mg/kg body weight/day) to canthaxanthin calculated based on existing MRLs of 15 mg canthaxanthin/kg liver (wet tissue) and 2.5 mg canthaxanthin/kg skin/fat (wet tissue).

Article Snippet: COMMISSION IMPLEMENTING REGULATION (EU) No 684/2014 of 20 June 2014 concerning the authorisation of canthaxanthin as a feed additive for breeder hens (holder of the authorisation DSM Nutritional products Ltd).

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure per population class, country and survey of consumers (mg/kg bw per day) to canthaxanthin based on existing MRLs for Carophyll Red 10% (2.5 mg/kg skin/fat, 15 mg/kg liver).

Article Snippet: COMMISSION IMPLEMENTING REGULATION (EU) No 684/2014 of 20 June 2014 concerning the authorisation of canthaxanthin as a feed additive for breeder hens (holder of the authorisation DSM Nutritional products Ltd).

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure to canthaxanthin (mg/kg body weight/day) calculated based on existing MRLs in poultry and salmonids.

Article Snippet: COMMISSION IMPLEMENTING REGULATION (EU) No 684/2014 of 20 June 2014 concerning the authorisation of canthaxanthin as a feed additive for breeder hens (holder of the authorisation DSM Nutritional products Ltd).

Techniques:

Journal: EFSA Journal

Article Title: Assessment of the feed additive consisting of canthaxanthin (Carophyll® Red 10%) for breeder hens for the renewal of its authorisation ( DSM Nutritional Products Ltd.)

doi: 10.2903/j.efsa.2025.9253

Figure Lengend Snippet: Chronic dietary exposure per population class, country and survey of consumers (mg/kg bw per day) to canthaxanthin calculated based on existing MRLs in poultry (2.5 mg/kg skin/fat, 15 mg/kg liver, 8.1 mg/kg whole egg) and existing MRL in trout/salmon (10 mg/kg muscle).

Article Snippet: COMMISSION IMPLEMENTING REGULATION (EU) No 684/2014 of 20 June 2014 concerning the authorisation of canthaxanthin as a feed additive for breeder hens (holder of the authorisation DSM Nutritional products Ltd).

Techniques:

Journal: The Plant Journal

Article Title: Chlamydomonas reinhardtii , Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores

doi: 10.1111/tpj.17261

Figure Lengend Snippet: (a) HPLC analysis (Gradient I) of total pigment extracts from haploid vegetative cells (strain CC‐620) and diploid zygospores of Chlamydomonas reinhardtii after maturation in the dark for 3 months (in boxed part of chromatogram, y‐axis zoomed in 20‐fold), and saponification products from either fraction I containing the putative monoacyl esters or fraction II containing the putative diacyl esters of the ketocarotenoids from the zygospore extract. (b) Biosynthetic scheme illustrating the postulated zygospore‐specific pathway from photosynthetic carotenoids to ketocarotenoids indicated by arrows and enzyme names in red and names of zygospore‐specific pigments italicized. The pathway impaired in the lor1 mutant is indicated by arrows with broken lines. In the chemical structures of the three major ketocarotenoids from the zygospores, blue arrows label the hydroxyl groups at C3 of the β‐ionone rings that can be esterified with fatty acids. Positions of the C‐atoms 2–7 and 2′–7′ are exemplified for canthaxanthin. (c) HPLC analysis (Gradient II) of ketocarotenoid acyl esters in C reinhardtii zygospores (22 days) resulting from homozygous crosses of either wild type (upper trace) or the lor1 ‐mutant (lower trace) which is deficient in α‐carotene and the xanthophylls derived thereof. Chromatograms were normalized to the β‐carotene peak. Dashed lines connect peaks of astaxanthin acyl esters present in both strains. A, trans ‐astaxanthin acyl ester; a , 13‐ cis ‐astaxanthin acyl ester; Asta, astaxanthin; β, β‐ionone ring; BKT, β‐carotene ketolase; Cantha, canthaxanthin; Car, carotene; Chl, chlorophyll; Chl a ′, Chlorophyll a epimer; CVDE, chlorophycean violaxanthin de‐epoxidase; ε, ε‐ionone ring; K, trans ‐4‐ketolutein acyl ester; k, 13(′) ‐cis ‐4‐ketolutein acyl ester; Ketolut, 4‐ketolutein; Loro, loroxanthin; Lut, lutein; Neo, neoxanthin; Vio, violaxanthin; ZEP, zeaxanthin epoxidase.

Article Snippet: The reference pigments used were synthetic astaxanthin (racemic mixture of three possible isomers at the C3 and C3′ hydroxyl groups) and canthaxanthin (both kindly donated by BASF) and natural astaxanthin and canthaxanthin from H. pluvialis .

Techniques: Mutagenesis, Derivative Assay

Journal: The Plant Journal

Article Title: Chlamydomonas reinhardtii , Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores

doi: 10.1111/tpj.17261

Figure Lengend Snippet: Activity of BKT from Chlamydomonas reinhardtii after heterologous expression in carotenogenic Escherichia coli strains supplying either (a) α‐carotene (aC) and β‐carotene (bC) or (b) lutein (L), 3′‐oxolutein (Oxl), zeinoxanthin (Zei) and zeaxanthin (Z) as substrates. Carotenoid concentrations in bacterial suspensions are shown at the start of induction (0 min) and after 45 min of growth in the absence (45 min control) or presence (45 min induced) of 0.04% arabinose. Abbreviations of ketolated products are: A, astaxanthin; Cn, canthaxanthin; Ec, echinenone; KaC, 4‐keto‐α‐carotene; KL, 4‐ketolutein; KOxl, 4‐keto‐3′‐oxolutein; KZei, 4‐ketozeinoxanthin. Data are average values of 4 biological replicates with error bars denoting standard deviations.

Article Snippet: The reference pigments used were synthetic astaxanthin (racemic mixture of three possible isomers at the C3 and C3′ hydroxyl groups) and canthaxanthin (both kindly donated by BASF) and natural astaxanthin and canthaxanthin from H. pluvialis .

Techniques: Activity Assay, Expressing, Control

Journal: The Plant Journal

Article Title: Chlamydomonas reinhardtii , Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores

doi: 10.1111/tpj.17261

Figure Lengend Snippet: HPLC analysis (Gradient I) of total pigment extracts from diploid zygospores (33 days) of Chlamydomonas reinhardtii ( C.r .), diploid zygospores (60 days) of Volvox carteri ( V.c .), and haploid aplanospores (35 days) of Haematococcus pluvialis ( H.p .). (a) Full chromatograms, (b) detail showing putative ketocarotenoid monoacyl esters, and (c) detail showing putative ketocarotenoid diacyl esters. Pigment abbreviations are: A, trans ‐astaxanthin acyl esters; a , 13‐ cis ‐astaxanthin acyl esters; Asta, astaxanthin; Cantha, canthaxanthin; Car, carotene; Chl, chlorophyll; K, trans ‐4‐ketolutein acyl esters; k , 13(′)‐ cis ‐4‐ketolutein acyl esters; Ketolut, 4‐ketolutein; Loro, loroxanthin; Lut, lutein; Neo, neoxanthin; Vio, violaxanthin.

Article Snippet: The reference pigments used were synthetic astaxanthin (racemic mixture of three possible isomers at the C3 and C3′ hydroxyl groups) and canthaxanthin (both kindly donated by BASF) and natural astaxanthin and canthaxanthin from H. pluvialis .

Techniques: