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NAD induces myeloid differentiation in K562. ( a ) <t>Wright</t> <t>Giemsa</t> staining showing morphological changes between NAD-treated and control cells after four days. The figure is representative of five independent experiments ( b ) Representative image showing the increase in the surface markers CD15, CD14 and CD11b upon NAD treatment by flow cytometry. The table reports the mean % ±SD of CD14 + , CD15 + , CD11b + cells of five independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. vehicle ( n = 5) ( c ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. The pictures are representative of five independent experiments ( d ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of three biological replicates ( n = 3). Error bars indicate ± s.d.
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NAD induces myeloid differentiation in K562. ( a ) <t>Wright</t> <t>Giemsa</t> staining showing morphological changes between NAD-treated and control cells after four days. The figure is representative of five independent experiments ( b ) Representative image showing the increase in the surface markers CD15, CD14 and CD11b upon NAD treatment by flow cytometry. The table reports the mean % ±SD of CD14 + , CD15 + , CD11b + cells of five independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. vehicle ( n = 5) ( c ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. The pictures are representative of five independent experiments ( d ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of three biological replicates ( n = 3). Error bars indicate ± s.d.
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NAD induces myeloid differentiation in K562. ( a ) <t>Wright</t> <t>Giemsa</t> staining showing morphological changes between NAD-treated and control cells after four days. The figure is representative of five independent experiments ( b ) Representative image showing the increase in the surface markers CD15, CD14 and CD11b upon NAD treatment by flow cytometry. The table reports the mean % ±SD of CD14 + , CD15 + , CD11b + cells of five independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. vehicle ( n = 5) ( c ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. The pictures are representative of five independent experiments ( d ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of three biological replicates ( n = 3). Error bars indicate ± s.d.
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Average 90 stars, based on 1 article reviews
camco quik stain kit - by Bioz Stars, 2026-09
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NAD induces myeloid differentiation in K562. ( a ) Wright Giemsa staining showing morphological changes between NAD-treated and control cells after four days. The figure is representative of five independent experiments ( b ) Representative image showing the increase in the surface markers CD15, CD14 and CD11b upon NAD treatment by flow cytometry. The table reports the mean % ±SD of CD14 + , CD15 + , CD11b + cells of five independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. vehicle ( n = 5) ( c ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. The pictures are representative of five independent experiments ( d ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of three biological replicates ( n = 3). Error bars indicate ± s.d.

Journal: Cells

Article Title: NAD Modulates DNA Methylation and Cell Differentiation

doi: 10.3390/cells10112986

Figure Lengend Snippet: NAD induces myeloid differentiation in K562. ( a ) Wright Giemsa staining showing morphological changes between NAD-treated and control cells after four days. The figure is representative of five independent experiments ( b ) Representative image showing the increase in the surface markers CD15, CD14 and CD11b upon NAD treatment by flow cytometry. The table reports the mean % ±SD of CD14 + , CD15 + , CD11b + cells of five independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. vehicle ( n = 5) ( c ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. The pictures are representative of five independent experiments ( d ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of three biological replicates ( n = 3). Error bars indicate ± s.d.

Article Snippet: The cells were then stained with the Wright Giemsa solutions kit (CAMCO STAIN PAK Fort Lauderdale, FL, USA, pc#702) according to manufacturer’s instructions.

Techniques: Staining, Control, Flow Cytometry