Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on the intestinal physical barrier function in C. perfringens -challenged mouse. ( A ) Intestinal damage status. ( B ) Body weight change. # p < 0.05; * p < 0.05. ( C , D ) Histomorphology of the jejunum. ( E ) TEM of the jejunum microvilli. TJ, tight junction. MV, microvilli length. Scale bars, 200 μm. Means ± SD (n = 6). a–c Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques:

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on the expression of jejunum tight junction proteins and serum intestinal barrier markers in C. perfringens -challenged mouse. ( A ) The content of DAO, D-LA and Endotoxin. ( B ) The gene expression of Claudin-1 , Claudin-2 , Claudin-5 , Occludin and ZO-1 . Means ± SD (n = 6). a–d Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Expressing, Gene Expression

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on the content of cytokines and antioxidant indicators in C. perfringens -challenged mouse. ( A ) F4/80 and Arg1 immunofluorescence analysis in the jejunum. ( B ) the content of TNF-α, IL-1β, IL-8 and IL-6. Scale bars, 100 μm. Means ± SD (n = 6). a–c Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Immunofluorescence

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on the content of antioxidant indicators in C. perfringens -challenged mouse. ( A , B ) Serum and jejunum antioxidant indicators (T-AOC, T-SOD, CAT and MDA). Scale bars, 100 μm. Means ± SD (n = 6). a–c Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques:

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on jejunum apoptosis status in C. perfringens -challenged mouse. ( A ) Ki67 immunofluorescence analysis in the jejunum. ( B ) TUNEL immunofluorescence analysis in the jejunum. Scale bars, 100 μm. Means ± SD (n = 6). a–c Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Immunofluorescence, TUNEL Assay

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on jejunum stem cell proliferation and differentiation of C. perfringens -challenged mouse. ( A , B ) Immunofluorescence analysis of LYZ and MUC2. ( C ) the relative mRNA of Intestinal stem cell marker genes ( Lgr5 , Sox-9 and Olfm4 ). ( D ) the relative mRNA of Paneth cell marker genes ( LYZ and MMP7 ) and goblet cell marker genes ( MUC2 , ATOH1 and Spink4 ). Scale bars, 100 μm. Means ± SD (n = 6). a–d Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Immunofluorescence, Marker

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of SC06 on the activation of the Wnt/β-catenin signaling pathway in C. perfringens -challenged mouse. ( A ) Immunofluorescence analysis of β-catenin in jejunum. Scale bars, 100 μm. ( B ) Western blot analysis of β-catenin, Cyclin-D1, and C-myc protein expression in jejunum. Means ± SD (n = 6). ( C ) Relative mRNA expression of Wnt/β-catenin signaling-related genes, including β-catenin, Cyclin-D1, AXIN, Wnt3, BMI-1, LRP5, LRP6, and FZD7, as determined by qPCR. a–d Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Activation Assay, Immunofluorescence, Western Blot, Expressing

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: SC06 upregulated intestinal organoid proliferation under C. perfringens -challenge. ( A ) Crypts from small intestines were seeded onto Matrigel and cultured for 5 days to obtain well-developed organoids. ( B ) Organoids were treated with or without SC06 (1 × 10 8 CFU per well) for 24 h, C. perfringen (1 × 10 8 CFU per well) was utilized to create an inflammatory model for 6 h; Scale bars, 100 μm; ( C ) The surface area, forming efficiency and building efficiency of organoids was calculated. ( D ) he relative mRNA of C-myc , PCNA and Ki67 . Means ± SD (n = 6). a–d Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Cell Culture

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: SC06 upregulated intestinal organoid differentiation under C. perfringens -challenge. ( A ) the relative mRNA of stem cell marker ( Lgr5 , Sox-9 and Olfm4 ). ( B ) the relative mRNA of Paneth cell marker genes ( LYZ and MMP7 ) ( C ) the relative mRNA of goblet cell marker ( MUC2 , ATOH1 , and Spink4 ). Means ± SD (n = 6). a–d Means within a row with various superscripts vary substantially ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Marker

Journal: Microorganisms

Article Title: Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens -Challenged Mouse

doi: 10.3390/microorganisms13092136

Figure Lengend Snippet: Effects of Wnt inhibition on the protective role of SC06 in intestinal organoids challenged with C. perfringens. SC06 + CP + Wnt-C59: Pre-treated with 100 nM Wnt-C59 for 24 h before SC06 and CP. ( A ) Representative images of intestinal organoids from the SC06 + CP group and the SC06 + CP + Wnt inhibitor group. Scale bars, 100 µm. Quantitative analysis showed that budding efficiency, surface area, and forming efficiency were significantly reduced following Wnt inhibition compared with SC06 + CP. ( B ) Quantification of organoid forming efficiency and relative mRNA expression of Wnt/β-catenin signaling–related genes ( β-catenin , Cyclin -D1, Wnt3 , Axin , BMI-1 , LRP5 , and LRP6 ). Data are presented as means ± SD (n = 6). a,b Means within a row with different superscripts differ significantly ( p < 0.05).

Article Snippet: C. perfringens type A (ATCC 13124) was cultured in Reinforced clostridium medium (RCM; Hopebio, Qingdao, China) and incubated at 37 °C under anaerobic conditions for 18 h. The bacterial cultures that had been incubated overnight were centrifuged at 5000× g for 5 min.

Techniques: Inhibition, Expressing

Journal: IDCases

Article Title: An autopsy case of gas gangrene, massive intravascular hemolysis, and cytokine storm due to Clostridium perfringens type A infection

doi: 10.1016/j.idcr.2024.e02085

Figure Lengend Snippet: Patient with lethal Clostridium perfringens infection with massive intravascular hemolysis and gas gangrene. (a) Serum of the patient at presentation. (b) Abdominal computed tomography (CT) images obtained at presentation (left panel), 1.5 h (middle panel), and 2.5 h after death (right panel). The lesion in the right lobe of the liver, initially seen as a low-density area at presentation (left panel), was replaced by a gas-filled cavity 1.5 h later (middle panel). A postmortem CT scan revealed rapid and massive expansion of gas-filled cavities in the right and left lobes of the liver (right panel).

Article Snippet: Genomic PCRs targeting CPA, CPB, ETX, ITX, CPE, NetB, PFO, and ColA were performed using bacterial DNA of C. perfringens isolated from the patient’s blood and a commercially available type A strain of C. perfringens (JCM#1290; RIKEN BioResource Research Center, Tsukuba, Japan).

Techniques: Infection, Computed Tomography

Journal: IDCases

Article Title: An autopsy case of gas gangrene, massive intravascular hemolysis, and cytokine storm due to Clostridium perfringens type A infection

doi: 10.1016/j.idcr.2024.e02085

Figure Lengend Snippet: Host cytokine responses against Clostridium perfringens isolated from this case. (a) Toxin profiles of Clostridium perfringens isolated from the patient and a commercially available type A strain (JCM#1290). The leftmost lane represents the 100-bp DNA ladder. Agarose gel electrophoresis of polymerase chain reaction (PCR) products revealed that C. perfringens isolated from the patient expressed CPA, PFO, and ColA, but not CPE. CPA, C. perfringens α toxin; PFO, perfringolysin O; ColA, collagenase. (b) Profiles of serum cytokines and chemokines. Cytokine and chemokine arrays revealed heightened proinflammatory responses in the patient serum. CCL2; C-C chemokine ligand 2, CXCL8; C-X-C motif chemokine ligand 8, G-CSF; granulocyte-colony stimulating factor. (c) Toll-like receptors (TLRs) involved in the production of proinflammatory cytokines by C. perfringens . Splenocytes prepared from C57BL/6 mice or mice deficient in TLR2 and/or TLR4 were stimulated with heat-killed C. perfringens . IL-6 mRNA expression was expressed as the mean ± standard error of the mean. * * P < 0.01.

Article Snippet: Genomic PCRs targeting CPA, CPB, ETX, ITX, CPE, NetB, PFO, and ColA were performed using bacterial DNA of C. perfringens isolated from the patient’s blood and a commercially available type A strain of C. perfringens (JCM#1290; RIKEN BioResource Research Center, Tsukuba, Japan).

Techniques: Isolation, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Expressing