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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
Female Balb C Strain Code 028 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Wolters Kluwer Health modifier variant (designated m1) in the coding region of apol1 (c.792c>a; p.asn264lys (rs73885316)
Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to <t>H1ssF-Cys.</t> <t>BALB/c</t> mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.
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Image Search Results


Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to H1ssF-Cys. BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.

Journal: Cell Reports Medicine

Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys

doi: 10.1016/j.xcrm.2026.102746

Figure Lengend Snippet: Conjugation of R848 to H1ssF-Cys nanoparticles (A) Representative image of H1ssF-Cys mutant. (B and C) Size (B) and PDI (C) analysis of H1ssF (WT and Cys mutant) using DLS. (D) Schematic for conjugation of R848 to H1ssF-Cys. BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. (E) BALB/c mice ( n = 3–12 across 3–4 experiments depending on the vaccine tested) were vaccinated and NC99 stem-specific Abs were quantified 14 days p.v. The dotted line indicates the limit of detection for the assay. TT was defined as the highest dilution with an OD 450 greater than three times the assay background. Lines represent the mean ± SEM. (F) Negatively stained TEM images of the conjugated H1ssF-R848. The scale bar lengths are as follows: 50 nm (A and F, left), 10 nm (F, right). Not significant p ≥ 0.05 (not indicated on graph), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001.

Article Snippet: Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.

Techniques: Conjugation Assay, Mutagenesis, Staining