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Journal: JBMR Plus
Article Title: Transcriptional profiling of otic capsule and femur in the oim mouse model of osteogenesis imperfecta
doi: 10.1093/jbmrpl/ziag088
Figure Lengend Snippet: Whole tissue transcriptomes of otic capsules and femora exhibit significant differences. (A) Principal component analysis depicts clear separation of RNA-seq data obtained from otic capsules and femora. (B) Top 6 transcript sets enriched in otic capsules (top) and femora (bottom) (logFC: log2 fold change, FDR: false-discovery rate). (C) Boxplots depict the expression of representative transcripts involved in neuron-associated pathways, lipid and steroid metabolism, BMP-, FGF-, and WNT pathways, and hematopoiesis. All measurements are clustered into femoral and otic capsule groups. (D) Heatmap of differentially expressed transcripts associated with WNT- and FGF-signaling, mucin- and keratin-production, as well as HOX-patterning and hematopoiesis (F: femur, O: otic capsule, WT, HET: heterozygous, HOM: homozygous, 12 W: 12-wk-old, 25 W: 25-wk-old, CPM: counts per million).
Article Snippet:
Techniques: Capsules, RNA Sequencing, Expressing
Journal: JBMR Plus
Article Title: Transcriptional profiling of otic capsule and femur in the oim mouse model of osteogenesis imperfecta
doi: 10.1093/jbmrpl/ziag088
Figure Lengend Snippet: Age affects the transcriptomes of mouse femora and otic capsules. (A) Volcano plot depicting significantly altered transcripts (FDR < 0.05, fold change >1.5, highlighted) in a comparison between RNA-seq libraries obtained from 12-wk-old and 25-wk-old mice, regardless of tissue type. Top gene sets with significant changes due to age are listed. (B) Volcano plots depicting age induced changes in femoral and otic capsule specimens separately. (C) Boxplots of representative transcripts associated with osteoblast signaling (Col1a1 and Bglap), extracellular matrix remodeling (Timp1), and autophagy (Pik3c3) that are significantly regulated by age. (F: femur, O: otic capsule, WT, HET: heterozygous, HOM: homozygous, 12 W: 12-wk-old, 25 W: 25-wk-old, CPM: counts per million).
Article Snippet:
Techniques: Capsules, Comparison, RNA Sequencing