Journal: Non-coding RNA Research

Article Title: LncRNA-EME1 enhances BRCA1 recruitment and alters repair of DNA damage in cervical cancer radioresistance

doi: 10.1016/j.ncrna.2025.09.006

Figure Lengend Snippet: Silenced lncRNA-EME1 destroyed the capacity of DNA repair in the post-irradiation cervical cancer cells. (A) γ-H2AX focus assay was used to detect the level of γ-H2AX in the post-irradiation HeLa-IR cells with lncRNA-EME1 silenced. (B) This bar graph shows the WB assay results that the protein expression changes associated with DNA break repair, including ATM, EME1, p53, and BRCA1 in the post-irradiation HeLa-IR cells with lncRNA-EME1 silenced. All experiments included appropriate controls were repeated at least three times to ensure reproducibility. The one-way ANOVA was used to evaluate the comparison among multiple groups. ∗∗ p < 0.01, ∗ p < 0.05.

Article Snippet: BRCA1 , Proteintech , 22362-1-AP , WB: 1:2000 IHC: 1:500 IP: 1:100.

Techniques: Irradiation, Expressing, Comparison

Journal: Non-coding RNA Research

Article Title: LncRNA-EME1 enhances BRCA1 recruitment and alters repair of DNA damage in cervical cancer radioresistance

doi: 10.1016/j.ncrna.2025.09.006

Figure Lengend Snippet: The upregulation of lncRNA-EME1 influenced the DNA damage and contributed to enhanced cell death. (A) γ-H2AX focus assay was used to display different intensities of fluorescence by detecting the level of γ-H2AX in HeLa cells with overexpression of lncRNA-EME, combined with ABT-888 treatment and subsequent 4Gy X-ray irradiation. The bar graph quantifies the severity of DNA damage. (B) The established I-SceI-HR system was used to detect the apoptosis rates. (C) WB assay showed the protein expression changes associated with DNA break repair including RAP80, p-CtIP, CtIP, and BRCA1. All experiments included appropriate controls were repeated at least three times to ensure reproducibility. The one-way ANOVA was used to evaluate the comparison among multiple groups. ∗∗ p < 0.01, ∗ p < 0.05.

Article Snippet: BRCA1 , Proteintech , 22362-1-AP , WB: 1:2000 IHC: 1:500 IP: 1:100.

Techniques: Fluorescence, Over Expression, Irradiation, Expressing, Comparison

Journal: Non-coding RNA Research

Article Title: LncRNA-EME1 enhances BRCA1 recruitment and alters repair of DNA damage in cervical cancer radioresistance

doi: 10.1016/j.ncrna.2025.09.006

Figure Lengend Snippet: LncRNA-EME1 interacted with BRCA1 to influence the malignant progression of cervical cancer. (A) The direct interaction between lncRNA-EME1 and BRCA1 was verified by RNA pull down using biotin-labeled lncRNA-EME1, followed by WB detection of BRAC1 in the precipitated complex. (B) The interaction between lncRNA-EME1 and BRCA1 was verified by RIP assay through BRAC1 antibody incubation, and the expression of lncRNA-EME1 in precipitated products was detected by RT-qPCR. (C) The WB assay detected the silencing efficiency of BRCA1 protein in HeLa-IR cells. (D–F) The apoptosis rates (D), clonogenic survival ability (E), and ROS levels (F) were evaluated in HeLa-IR cells after irradiation, with lncRNA-EME1 overexpression combined with BRCA1 silencing. All experiments included appropriate controls were repeated at least three times to ensure reproducibility. The one-way ANOVA was used to evaluate the comparison among multiple groups. ∗∗ p < 0.01, ∗ p < 0.05.

Article Snippet: BRCA1 , Proteintech , 22362-1-AP , WB: 1:2000 IHC: 1:500 IP: 1:100.

Techniques: Labeling, Incubation, Expressing, Quantitative RT-PCR, Irradiation, Over Expression, Comparison

Journal: Non-coding RNA Research

Article Title: LncRNA-EME1 enhances BRCA1 recruitment and alters repair of DNA damage in cervical cancer radioresistance

doi: 10.1016/j.ncrna.2025.09.006

Figure Lengend Snippet: LncRNA-EME1 interacted with BRCA1 to impact the DNA damage repair capacity. (A) γ-H2AX focus assay was used to display different intensities of fluorescence by detecting the level of γ-H2AX in HeLa cells with overexpression of lncRNA-EME, combined with BRCA1 silencing and subsequent 4 Gy X-ray irradiation. (B) The established I-SceI-HR system was used to detect the apoptosis rates. (C) WB assay showed the protein expression changes associated with DNA break repair. All experiments included appropriate controls were repeated at least three times to ensure reproducibility. The one-way ANOVA was used to evaluate the comparison among multiple groups. ∗∗ p < 0.01, ∗ p < 0.05.

Article Snippet: BRCA1 , Proteintech , 22362-1-AP , WB: 1:2000 IHC: 1:500 IP: 1:100.

Techniques: Fluorescence, Over Expression, Irradiation, Expressing, Comparison

Journal: Non-coding RNA Research

Article Title: LncRNA-EME1 enhances BRCA1 recruitment and alters repair of DNA damage in cervical cancer radioresistance

doi: 10.1016/j.ncrna.2025.09.006

Figure Lengend Snippet: lncRNA-EME1 silencing enhances the anti-tumor effect of irradiation in vivo through impaired DNA repair. (A) The xenograft tumors were excised and photographed after stripping from mice in four groups: Lenti-control, Lenti-sh-EME1, Lenti-control with irradiation, and Lenti-sh-EME1 with irradiation. (B–C) The volume (B) and weight (C) of the tumors were recorded at specified time points. (D) IHC assay was used to detect the BRCA1 expression within the tumor tissues. (E) TUNEL assay was performed to indicate the apoptotic effect in the tumor tissues. (F) The γ-H2AX focus assay was used to demonstrate the number of unrepaired DNA damage sites in the tumor tissues. All experiments included appropriate controls were repeated at least three times to ensure reproducibility. The one-way ANOVA was used to evaluate the comparison among multiple groups. ∗∗ p < 0.01, ∗ p < 0.05.

Article Snippet: BRCA1 , Proteintech , 22362-1-AP , WB: 1:2000 IHC: 1:500 IP: 1:100.

Techniques: Irradiation, In Vivo, Stripping Membranes, Control, Expressing, TUNEL Assay, Comparison