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Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
Intratracheal Instillation, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
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intratracheal instillation  (MedChemExpress)
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MedChemExpress intratracheal instillation
Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
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bleomycin sulfate  (MedChemExpress)
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Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
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Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
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hy b0579 bleomycin sulfate med chem express  (MedChemExpress)
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Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
Hy B0579 Bleomycin Sulfate Med Chem Express, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bleomycin+sulfate/Cyclosporin+A/pm42360879-525-17-20
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bleomycin sulfate  (Thermo Fisher)
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Thermo Fisher bleomycin sulfate
Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
Bleomycin Sulfate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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200 bleomycin sulfate macklin cat  (Macklin Inc)
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Macklin Inc 200 bleomycin sulfate macklin cat
Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
200 Bleomycin Sulfate Macklin Cat, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bleomycin antibiotic  (Thermo Fisher)
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Thermo Fisher bleomycin antibiotic
Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
Bleomycin Antibiotic, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bleomycin+sulfate/Bleomycin+sulfate%2C+Cell+Culture+Grade/pm42248856-128-10-13
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bleomycin  (Selleck Chemicals)
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Selleck Chemicals bleomycin
Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following <t>bleomycin</t> injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.
Bleomycin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bleomycin+sulfate/Bleomycin+Sulfate/pmc12859463-384-4-11
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Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following bleomycin injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.

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Figure Lengend Snippet: Engraftment and functional assessment of transplanted alveolar progenitor‐like organoids (APLOs) in a murine model of lung injury. (A) Schematic diagram of the experimental timeline for the transplantation and analysis of APLOs in a mouse xenograft model. Created in BioRender. Ma, H. (2026) https://BioRender.com/snq201j (B) Immunofluorescence staining of mouse distal lung sections at 3 days post‐transplantation (3 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 25 µm (left panels), 12.5 µm (right panel). (C) Immunofluorescence staining of mouse distal lung sections at 7 days post‐transplantation (7 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (D) Immunofluorescence staining of mouse distal lung sections at 21 days post‐transplantation (21 dpt), showing donor‐derived human cells identified by HuLamin A+C (green) and co‐stained for the pulmonary lineage markers proSFTPC (white) and PDPN (red). Nuclei were counterstained with DAPI. Scale bars, 50 µm (left panels), 10 µm (right panel). (E) Quantification of the HuLamin + transplanted area in recipient lungs at 3, 7, and 21 days post‐transplantation. Data are presented as mean ± SD (n = 3). Statistical comparisons were performed among the indicated time points; ns, not significant; * p < 0.05. (F) Arterial blood oxygen saturation (SO 2 %) measured at indicated time points following bleomycin injury in mice treated with APLO‐derived cells or PBS control. Data are presented as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.

Article Snippet: Lung injury was induced via intratracheal instillation of bleomycin (TargetMol, T6116) at a dose of 1.5 U/kg body weight in 30 μL sterile PBS.

Techniques: Functional Assay, Organoids, Transplantation Assay, Analysis, Xenograft Assay, Immunofluorescence, Staining, Derivative Assay, Control