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Differential abundance data analysis of AMR genes from samples sourced at WWTWs. Data were compared following stratification by season. Summer (included the months of December, January, February), autumn (included the months of March, April, May), winter (included the months of June, July, August) and spring (included the months of September, October, November).

Journal: PLOS ONE

Article Title: Metagenomics analysis of sewage for surveillance of antimicrobial resistance in South Africa

doi: 10.1371/journal.pone.0309409

Figure Lengend Snippet: Differential abundance data analysis of AMR genes from samples sourced at WWTWs. Data were compared following stratification by season. Summer (included the months of December, January, February), autumn (included the months of March, April, May), winter (included the months of June, July, August) and spring (included the months of September, October, November).

Article Snippet: In total, significant seasonal abundance differences were found for only six AMR genes, which included: bla KPC-2 beta-lactamase gene (AY034847), bla KPC-34 beta-lactamase gene (KU985429), bla OXA-334 beta-lactamase gene (KF203108), all encoding resistance to carbapenems; bla NPS-1 beta-lactamase gene (AY027589) encoding resistance to beta-lactams; aac(6’)-Ib-Hangzhou gene (FJ503047), aac(3)-Ia gene (X15852), both encoding resistance to aminoglycosides ( , ).

Techniques:

Differential abundance data analysis of AMR genes from samples sourced at WWTWs. Data were compared following stratification by season. Summer (included the months of December, January, February), autumn (included the months of March, April, May), winter (included the months of June, July, August) and spring (included the months of September, October, November).

Journal: PLOS ONE

Article Title: Metagenomics analysis of sewage for surveillance of antimicrobial resistance in South Africa

doi: 10.1371/journal.pone.0309409

Figure Lengend Snippet: Differential abundance data analysis of AMR genes from samples sourced at WWTWs. Data were compared following stratification by season. Summer (included the months of December, January, February), autumn (included the months of March, April, May), winter (included the months of June, July, August) and spring (included the months of September, October, November).

Article Snippet: Summer compared to Spring , bla KPC-2 gene (AY034847) more abundant in Spring; aac(6’)-Ib-Hangzhou gene (FJ503047) more abundant in Spring , No significant abundance difference in AMR genes , No significant abundance difference in AMR genes , No significant abundance difference in AMR genes.

Techniques:

Susceptibility testing results for Ambler position D179 site-saturation bla KPC-2 mutants expressed in E. coli DH10B.

Journal: Antibiotics

Article Title: The Effectiveness of Imipenem–Relebactam against Ceftazidime-Avibactam Resistant Variants of the KPC-2 β-Lactamase

doi: 10.3390/antibiotics12050892

Figure Lengend Snippet: Susceptibility testing results for Ambler position D179 site-saturation bla KPC-2 mutants expressed in E. coli DH10B.

Article Snippet: Due to technical difficulties, the bla KPC-2 D179E, D179I, or D179S genes were purchased from Celtek Genes Service a subdivision of Celtek Bioscience, LLC (Franklin, TN, USA), and cloned into the pBC SK(+) phagemid, as previously described [ ].

Techniques: