Journal: Acta biomaterialia

Article Title: Single-dose combination nanovaccine induces both rapid and long-lived protection against pneumonic plague

doi: 10.1016/j.actbio.2019.10.016

Figure Lengend Snippet: Serum samples collected from C57BL/6NCrl mice (n = 12–16 per group) immunized with either saline, CDN Nanovaccine, Nanovaccine, or Combination Nanovaccine at 14, 79, and 178 DPI was analyzed for total anti-F1-V IgG antibodies against twenty-seven 14- to 17-mer linear peptides (11 amino acid overlaps) from the F1 antigen and fifty-three 15- to 17-mer linear peptides (11 or 12 amino acid overlaps) from the V antigen. The peptides were covalently bound to microarray slides as described in Materials and Methods. Each row corresponds to a specific peptide, the top row representing peptide F1 and the proceeding downward rows corresponding to each following linear peptide incrementally through peptide V53. Each column represents responses from a single mouse. The mean fluorescence intensity of serum responses to each peptide is represented by a range of color from white (no response) to purple (maximum response). The full-length F1-V fusion protein was used as a positive control, and Bacillus anthracis protective antigen (PA) and chicken egg ovalbumin (OVA) were used as negative controls. Black arrows indicate significance (p ≤ 0.05) of Combination Nanovaccine and CDN Vaccine serum compared to naïve serum at all time points evaluated. Blue arrows indicate significance (p < –0.05) of Combination Nanovaccine and CDN Vaccine serum compared to naïve serum at 179 DPI. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Peptide microarray printing and analysis Twenty-seven 14- to 17-mer linear peptides (11 amino acid overlaps) spanning the full length of F1 antigen and fifty-three 15-to 17-mer linear peptides (11 or 12 amino acid overlaps) spanning the full length of V antigen, as well as full length proteins F1-V, Bacillus anthracis protective antigen (PA), and chicken egg ovalbumin (OVA) were printed onto Nexterion Slide AL (Schott, Louisville, KY) using a BioRobotics MicroGRID II microarray printer (Genomic Solutions, Inc. Ann Arbor, MI).

Techniques: Microarray, Fluorescence, Positive Control