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A, Effect of WIPTIDE (WIP; 1 μg; n = 12 paws), <t>BIMM</t> (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) on the baseline (BL) paw threshold (grams ± SEM) of ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars;n = 12 paws) rats. *p < 0.0001 (ANOVA, followed by Fisher's PLSD post hoc test).NS, Not statistically significant. B, Reduction in lowered paw-withdrawal threshold (hyperalgesia) produced by WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) plotted as percentage change in nociceptive threshold in ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars; n = 12 paws) rats. *p < 0.0001 (one-way ANOVA and Fisher's PLSD post hoc test). NS, Not statistically significant.
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A, Effect of WIPTIDE (WIP; 1 μg; n = 12 paws), <t>BIMM</t> (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) on the baseline (BL) paw threshold (grams ± SEM) of ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars;n = 12 paws) rats. *p < 0.0001 (ANOVA, followed by Fisher's PLSD post hoc test).NS, Not statistically significant. B, Reduction in lowered paw-withdrawal threshold (hyperalgesia) produced by WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) plotted as percentage change in nociceptive threshold in ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars; n = 12 paws) rats. *p < 0.0001 (one-way ANOVA and Fisher's PLSD post hoc test). NS, Not statistically significant.
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A, Effect of WIPTIDE (WIP; 1 μg; n = 12 paws), <t>BIMM</t> (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) on the baseline (BL) paw threshold (grams ± SEM) of ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars;n = 12 paws) rats. *p < 0.0001 (ANOVA, followed by Fisher's PLSD post hoc test).NS, Not statistically significant. B, Reduction in lowered paw-withdrawal threshold (hyperalgesia) produced by WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) plotted as percentage change in nociceptive threshold in ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars; n = 12 paws) rats. *p < 0.0001 (one-way ANOVA and Fisher's PLSD post hoc test). NS, Not statistically significant.
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A, Effect of WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) on the baseline (BL) paw threshold (grams ± SEM) of ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars;n = 12 paws) rats. *p < 0.0001 (ANOVA, followed by Fisher's PLSD post hoc test).NS, Not statistically significant. B, Reduction in lowered paw-withdrawal threshold (hyperalgesia) produced by WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) plotted as percentage change in nociceptive threshold in ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars; n = 12 paws) rats. *p < 0.0001 (one-way ANOVA and Fisher's PLSD post hoc test). NS, Not statistically significant.

Journal: The Journal of Neuroscience

Article Title: Key Role for the Epsilon Isoform of Protein Kinase C in Painful Alcoholic Neuropathy in the Rat

doi: 10.1523/JNEUROSCI.20-22-08614.2000

Figure Lengend Snippet: A, Effect of WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) on the baseline (BL) paw threshold (grams ± SEM) of ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars;n = 12 paws) rats. *p < 0.0001 (ANOVA, followed by Fisher's PLSD post hoc test).NS, Not statistically significant. B, Reduction in lowered paw-withdrawal threshold (hyperalgesia) produced by WIPTIDE (WIP; 1 μg; n = 12 paws), BIMM (1 μg; n = 12 paws), and PKCε-I (1 μg; n = 12 paws) plotted as percentage change in nociceptive threshold in ethanol-fed (filled bars; n = 12 paws) and isocalorically fed control (hatched bars; n = 12 paws) rats. *p < 0.0001 (one-way ANOVA and Fisher's PLSD post hoc test). NS, Not statistically significant.

Article Snippet: Bisindolylmaleimide (BIMM) (nonspecific PKC inhibitor), PKCε-specific translocation inhibitor peptide (PKCε-I) ( Johnson et al., 1996 ; Khasar et al., 1999 ) (Calbiochem, La Jolla, CA), and Walsh inhibitor peptide (WIPTIDE) (PKA inhibitor; Peninsula Laboratories, Belmont, CA) were used.

Techniques: Control, Produced