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Fluorescence images and quantification of nanoparticle uptake in TNFα-activated ECs under the following conditions: (A) VCAM1 blockade, (B) <t>β1-integrin</t> blockade on nanoparticles, and (C) CPZ treatment. Red: IA@MoNP, MoNP, or bare NP; blue: DAPI-stained nuclei. Scale bar = 50 μm. (A–B) *p < 0.05 vs. IgG antibody; # p < 0.05 vs. MoNP. (C) *p < 0.05 vs. control ECs; # p < 0.05 vs. bare NP. Data are presented as mean ± SD from n = 3 independent experiments.
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Fluorescence images and quantification of nanoparticle uptake in TNFα-activated ECs under the following conditions: (A) VCAM1 blockade, (B) <t>β1-integrin</t> blockade on nanoparticles, and (C) CPZ treatment. Red: IA@MoNP, MoNP, or bare NP; blue: DAPI-stained nuclei. Scale bar = 50 μm. (A–B) *p < 0.05 vs. IgG antibody; # p < 0.05 vs. MoNP. (C) *p < 0.05 vs. control ECs; # p < 0.05 vs. bare NP. Data are presented as mean ± SD from n = 3 independent experiments.
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Image Search Results


Fluorescence images and quantification of nanoparticle uptake in TNFα-activated ECs under the following conditions: (A) VCAM1 blockade, (B) β1-integrin blockade on nanoparticles, and (C) CPZ treatment. Red: IA@MoNP, MoNP, or bare NP; blue: DAPI-stained nuclei. Scale bar = 50 μm. (A–B) *p < 0.05 vs. IgG antibody; # p < 0.05 vs. MoNP. (C) *p < 0.05 vs. control ECs; # p < 0.05 vs. bare NP. Data are presented as mean ± SD from n = 3 independent experiments.

Journal: bioRxiv

Article Title: Integrin Activation Enhances Lesion-Specific Targeting of Monocyte-Mimetic Nanoparticles in Atherosclerosis

doi: 10.64898/2026.03.04.707824

Figure Lengend Snippet: Fluorescence images and quantification of nanoparticle uptake in TNFα-activated ECs under the following conditions: (A) VCAM1 blockade, (B) β1-integrin blockade on nanoparticles, and (C) CPZ treatment. Red: IA@MoNP, MoNP, or bare NP; blue: DAPI-stained nuclei. Scale bar = 50 μm. (A–B) *p < 0.05 vs. IgG antibody; # p < 0.05 vs. MoNP. (C) *p < 0.05 vs. control ECs; # p < 0.05 vs. bare NP. Data are presented as mean ± SD from n = 3 independent experiments.

Article Snippet: Membrane proteins were assessed by Western blot using antibodies against CD11b (Cell Signaling #17800, 1:1000), Na + /K + ATPase (Cell Signaling #3010, 1:1000), α4-integrin (Invitrogen #PA5-20599, 1:1000), and β1-integrin (Cell Signaling #4706S, 1:1000).

Techniques: Fluorescence, Staining, Control

(A) Schematic illustration of the experimental design. (B) IVIS imaging and quantification of nanoparticle accumulation in partially ligated LCA of ApoE -/- mice. (C) Representative cross-sectional fluorescence images of the LCA. Red: IA@MoNP or MoNP; green: elastin fibers; blue: DAPI-stained nuclei. Scale bar = 100 μm. (D–E) IVIS analysis showing (D) biodistribution in major organs across nanoparticle formulations and (E) reduction of IA@MoNP signal in the LCA following β1-integrin blockade on nanoparticles. (B) *p < 0.05 vs. RCA; # p < 0.05 vs. MoNP; (E) *p < 0.05 vs. IgG antibody. Data are presented as mean ± SD from n = 4 mice each group.

Journal: bioRxiv

Article Title: Integrin Activation Enhances Lesion-Specific Targeting of Monocyte-Mimetic Nanoparticles in Atherosclerosis

doi: 10.64898/2026.03.04.707824

Figure Lengend Snippet: (A) Schematic illustration of the experimental design. (B) IVIS imaging and quantification of nanoparticle accumulation in partially ligated LCA of ApoE -/- mice. (C) Representative cross-sectional fluorescence images of the LCA. Red: IA@MoNP or MoNP; green: elastin fibers; blue: DAPI-stained nuclei. Scale bar = 100 μm. (D–E) IVIS analysis showing (D) biodistribution in major organs across nanoparticle formulations and (E) reduction of IA@MoNP signal in the LCA following β1-integrin blockade on nanoparticles. (B) *p < 0.05 vs. RCA; # p < 0.05 vs. MoNP; (E) *p < 0.05 vs. IgG antibody. Data are presented as mean ± SD from n = 4 mice each group.

Article Snippet: Membrane proteins were assessed by Western blot using antibodies against CD11b (Cell Signaling #17800, 1:1000), Na + /K + ATPase (Cell Signaling #3010, 1:1000), α4-integrin (Invitrogen #PA5-20599, 1:1000), and β1-integrin (Cell Signaling #4706S, 1:1000).

Techniques: Imaging, Fluorescence, Staining