Journal: IBRO Neuroscience Reports

Article Title: Pre-treatment with royal jelly, environmental enrichment, and their combination role in stress induced neurobehavioral deficits in male rats

doi: 10.1016/j.ibneur.2026.03.012

Figure Lengend Snippet: Experimental Timeline: Rats were treated with EE, RJ, and EE + RJ for 14 consecutive days. Stress was induced on days 8–14. Behavioral tests were conducted on days 15–18. On day 19, the animals were decapitated to assess serum corticosterone and hippocampal BDNF levels.

Article Snippet: The evaluation of serum corticosterone levels and hippocampal BDNF levels was conducted using an enzyme-linked immunosorbent assay (ELISA) with a commercially available ELISA kit (E20160505043, Hangzhou Eastbiopharm Co. Ltd., Zhejiang Province, China) and rat BDNF ELISA kits from Boster Biological Technology Co. with a sensitivity limit of 4 pg/ml following the instructions provided by the manufacturer.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Pre-treatment with royal jelly, environmental enrichment, and their combination role in stress induced neurobehavioral deficits in male rats

doi: 10.1016/j.ibneur.2026.03.012

Figure Lengend Snippet: The effects of EE and RJ treatment on the hippocampal BDNF expression. The BDNF levels in the stressed group were significantly decreased than in the control group. EE and RJ treatment increased BDNF levels in the stressed rats. *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001 v.s. the Non-stressed control group, respectively; ^^ and ^^^ represent p < 0.01 and p < 0.001 v.s. the Non-treatment stressed group, respectively.

Article Snippet: The evaluation of serum corticosterone levels and hippocampal BDNF levels was conducted using an enzyme-linked immunosorbent assay (ELISA) with a commercially available ELISA kit (E20160505043, Hangzhou Eastbiopharm Co. Ltd., Zhejiang Province, China) and rat BDNF ELISA kits from Boster Biological Technology Co. with a sensitivity limit of 4 pg/ml following the instructions provided by the manufacturer.

Techniques: Expressing, Control

Journal: Cell Reports Medicine

Article Title: BDNF insufficiency exacerbates ALS progression

doi: 10.1016/j.xcrm.2026.102758

Figure Lengend Snippet: Therapeutic benefits of boosting BDNF signal in SOD1 G93A and TDP43 A315T mice with B90-1 (A) Treatment timeline for the SOD1 G93A and TDP43 A315T mice. B90-1 was administered through intravenous injection, and IgG was used as the control. Riluzole was provided per oral with separate vehicle controls. (B and E) Survival curves of SOD1 G93A (B) or TDP43 A315T (E) mice with different treatment. n = 12 mice for each group. Kaplan-Meier analysis was used for statistical comparison. (C and F) Motor function was measured by wire hang test (SOD1 G93A in C and TDP43 A315T in F) at different ages. n = 12 mice for each group to begin with more than 4 mice in each group for the last time point. Data are represented as mean ± SD. Two-way ANOVA with Tukey’s post hoc analysis was used for statistical comparison for the main group effect. (D and G) Quantification of ChAT-positive motor neurons in sections at lumbar L4–6 segments of 16-week-old SOD1 G93A (D) or TDP43 A315T (G) mice with different treatment. n = 3 mice for each group for SOD1 G93A and 6 mice for TDP43 A315T . Data are represented as mean ± SD. Student’s t tests were used for statistical comparisons.

Article Snippet: Bdnf +/−, SOD1G93A and TDP43 were acquired from Jackson Laboratory, Strain #: 002266, 002726 and 010700, respectively.

Techniques: Injection, Control, Comparison