Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 1. TT upregulated the BDNF/TrkB-CREB signalling pathway and prevented the loss of TJ and AJ proteins following SCI. (A–H) Representative western blots and quantification data for BDNF/β-Actin, PI3K/β-Actin, p-TrkB/TrkB, p-AKT/AKT and p-CREB/CREB in each group, columns represent the mean ± SD, n = 5. (I) Double staining for Claudin-5, Occludin, p120-Catenin, and β-Catenin/CD31/Hoechst. Red: Claudin-5/Occludin/p120-Catenin/β-Catenin; green: CD31; blue: Hoechst. Scale bar, 20 μm #p < 0.05 for the M group versus the S group, *p < 0.05 for the TM group versus the M group. (#p, *p < 0.05; ##p, **p < 0.01; ###p, ***p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Western Blot, Double Staining

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 2. The inhibitor downregulated the BDNF/TrkB-CREB signalling pathway and increased the permeability of the spinal cord, accompanied by poor motor function recovery. (A–H) Representative western blots and quantification data of BDNF/β-Actin, PI3K/β-Actin, p-TrkB/TrkB, p-AKT/AKT and p-CREB/CREB in the M and IM groups. Columns represent the mean ± SD, n = 5. (I) BBB scores in M, IM groups. (J, L) Representative quantification data of spinal cord water content in the M and IM groups; columns represent the mean ± SD, n = 5. (K) Representative Evans blue dye permeabilized into the spinal cord after SCI and (M) quantification of the amount of Evans blue (μg/g), n = 5. $p < 0.05 for the IM group versus the M group ($p < 0.05; $$p < 0.01; $$$p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Permeability, Western Blot

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 5. TT decreased endothelial permeability and improved functional recovery by upregulating the BDNF/TrkB-CREB signalling pathway. (A, C) Representative quantification data of spinal cord water content in the TM and ITM groups. Columns represent the mean ± SD, n = 5. &p < 0.05 for the ITM group versus the TM group (B, D) Representative Evans blue dye permeabilized into the spinal cord after SCI and quantification of the amount of Evans blue (μg/g), n = 5. (E) BBB scores in TM and ITM groups. (F) TEM shows the vascular EC-EC junctions in the TM and ITM. Arrows indicate an open tight junction and the scale bars are 0.5 μm. (G) Quantification of the TJ gap width between the TM and ITM; columns represent the mean ± SD. (H) Quantification of the TJ length between the TM and ITM; columns represent the mean ± SD. (&p < 0.05; &&p < 0.01; &&&p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Permeability, Functional Assay

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 6. TT inhibited BSCB disruption by upregulating the BDNF/TrkB-CREB signalling pathway. (A, B) Representative western blots and quantification data of TJ and AJ proteins in the TM and ITM groups. Columns represent the mean ± SD, n = 5. (C) Claudin- 5, Occludin, p120-Catenin, and β-Catenin/CD31/ Hoechst staining of sections from the spinal cord in the TM and ITM groups. Red: Claudin-5/Occludin/ p120-Catenin/β-Catenin; green: CD31; blue: Hoechst. Scale bar, 20 μm. (&p < 0.05; &&p < 0.01; &&&p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Disruption, Western Blot, Staining

Journal: Physiological Reports

Article Title: Multigenerational effects of fetal-neonatal iron deficiency on hippocampal BDNF signaling

doi: 10.1002/phy2.96

Figure Lengend Snippet: List of Real-Time PCR Taqman™ Probes

Article Snippet: BDNF-IV , NM_012513 , Rn02531967_s1 , Signaling molecule.

Techniques: Real-time Polymerase Chain Reaction, Membrane, Control

Journal: Physiological Reports

Article Title: Multigenerational effects of fetal-neonatal iron deficiency on hippocampal BDNF signaling

doi: 10.1002/phy2.96

Figure Lengend Snippet: Quantitative PCR (qPCR) analysis demonstrates increased expression of BDNF and downstream targets in the P15 F2 IS rats. Transcript levels were measured from the hippocampus of F1 iron sufficient (IS), F1 iron deficient (ID), and F2 iron-sufficient (F2 IS) rats for (A) BDNF-III and BDNF-IV, (B) trkB, and (C) Egr1, Hif1a, Cxcl12, and Dusp4. Values are means ± SEM; n = 4–6/group; * P < 0.05, ** P < 0.01, and *** P < 0.001, Unpaired t -test.

Article Snippet: BDNF-IV , NM_012513 , Rn02531967_s1 , Signaling molecule.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Physiological Reports

Article Title: Multigenerational effects of fetal-neonatal iron deficiency on hippocampal BDNF signaling

doi: 10.1002/phy2.96

Figure Lengend Snippet: Real-Time PCR Analysis of P65 Male Rat Hippocampus

Article Snippet: BDNF-IV , NM_012513 , Rn02531967_s1 , Signaling molecule.

Techniques: Real-time Polymerase Chain Reaction