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Regulation of PPS on the imbalance of intestinal microbiota in <t>T2DM</t> mice. All mice were sacrificed and colonic samples were collected for 16SrDNA gene sequencing. (A) Alpha diversity index-observed species. (B) Beta-diversity-NMDS. (C) Alteration of gut microbiota composition at family level. (D) Heatmap of genera level of each sample. (E) Phylogenetic tree of the genus level.
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Average 90 stars, based on 1 article reviews
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barcoded pyrosequencing of the v3-v4 region of 16s rdna gene  (Pyrosequencing Inc)
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Regulation of PPS on the imbalance of intestinal microbiota in <t>T2DM</t> mice. All mice were sacrificed and colonic samples were collected for 16SrDNA gene sequencing. (A) Alpha diversity index-observed species. (B) Beta-diversity-NMDS. (C) Alteration of gut microbiota composition at family level. (D) Heatmap of genera level of each sample. (E) Phylogenetic tree of the genus level.
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Regulation of PPS on the imbalance of intestinal microbiota in T2DM mice. All mice were sacrificed and colonic samples were collected for 16SrDNA gene sequencing. (A) Alpha diversity index-observed species. (B) Beta-diversity-NMDS. (C) Alteration of gut microbiota composition at family level. (D) Heatmap of genera level of each sample. (E) Phylogenetic tree of the genus level.

Journal: Frontiers in Nutrition

Article Title: Sequential Extraction, Characterization, and Analysis of Pumpkin Polysaccharides for Their Hypoglycemic Activities and Effects on Gut Microbiota in Mice

doi: 10.3389/fnut.2021.769181

Figure Lengend Snippet: Regulation of PPS on the imbalance of intestinal microbiota in T2DM mice. All mice were sacrificed and colonic samples were collected for 16SrDNA gene sequencing. (A) Alpha diversity index-observed species. (B) Beta-diversity-NMDS. (C) Alteration of gut microbiota composition at family level. (D) Heatmap of genera level of each sample. (E) Phylogenetic tree of the genus level.

Article Snippet: In order to evaluate the effect of pumpkin polysaccharides on the gut microbiota of T2DM mice, barcode pyrosequencing technology was used to analyze the structural changes in the gut microbiota of mice in the four groups.

Techniques: Sequencing