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Generation of Cricket Paralysis Virus (CrPV)-derived small interfering RNAs (siRNAs) with species-dependent length distribution. Length distribution of small RNAs mapping to CrPV, derived from fat body of CrPV-infected Spodoptera exigua ( A ), Locusta migratoria ( B ), and Bombus <t>terrestris</t> ( C ); as well as from CrPV-infected Trichoplusia ni High Five cells ( D ) and Tribolium castaneum TcA cells ( E ). Reads ( y axis): number of reads. Length ( x axis): length (nt) of the reads. Black: sense reads. Green: antisense reads. To avoid degradation products and general contaminants, reads shorter than 18 nt and longer than 31 nt were not included. Mapping was performed with Bowtie2 and graphs were obtained with the viRome package in R.
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Generation of Cricket Paralysis Virus (CrPV)-derived small interfering RNAs (siRNAs) with species-dependent length distribution. Length distribution of small RNAs mapping to CrPV, derived from fat body of CrPV-infected Spodoptera exigua ( A ), Locusta migratoria ( B ), and Bombus terrestris ( C ); as well as from CrPV-infected Trichoplusia ni High Five cells ( D ) and Tribolium castaneum TcA cells ( E ). Reads ( y axis): number of reads. Length ( x axis): length (nt) of the reads. Black: sense reads. Green: antisense reads. To avoid degradation products and general contaminants, reads shorter than 18 nt and longer than 31 nt were not included. Mapping was performed with Bowtie2 and graphs were obtained with the viRome package in R.

Journal: Viruses

Article Title: Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects

doi: 10.3390/v11080738

Figure Lengend Snippet: Generation of Cricket Paralysis Virus (CrPV)-derived small interfering RNAs (siRNAs) with species-dependent length distribution. Length distribution of small RNAs mapping to CrPV, derived from fat body of CrPV-infected Spodoptera exigua ( A ), Locusta migratoria ( B ), and Bombus terrestris ( C ); as well as from CrPV-infected Trichoplusia ni High Five cells ( D ) and Tribolium castaneum TcA cells ( E ). Reads ( y axis): number of reads. Length ( x axis): length (nt) of the reads. Black: sense reads. Green: antisense reads. To avoid degradation products and general contaminants, reads shorter than 18 nt and longer than 31 nt were not included. Mapping was performed with Bowtie2 and graphs were obtained with the viRome package in R.

Article Snippet: Bumblebee ( B. terrestris ) callow workers were obtained from Biobest (Westerlo, Belgium).

Techniques: Virus, Derivative Assay, Infection