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( A ) IgG (left) and IgA (right) reactivities of HC and HS3 serum pools towards healthy skin sections are shown. Scale bar: 100 µm. The white dotted line indicates the separation between epidermis (Epi) and dermis. ( B ) Same as in ( A ) towards healthy donor-derived KCs, with quantification of IgG signals normalized to Dapi. Scale bar: 50 µm. Mann–Whitney Statistical test, n = 3. ( C ) Dot plots illustrating the reactivity of HC and HS3 sera ( n = 11) against <t>peptidoglycan</t> (PGN), Keyhole limpet hemocyanin (KLH), MAPK14, lipopolysaccharide (LPS), double-stranded DNA (dsDNA), tubulin (TB), and lysozyme (LZ). Data were area under the curve (AUC) values determined by ELISA with serially diluted sera. Mann–Whitney statistical test. ( D ) Methodology for depleting HC and HS3 serum pools on bacteria (top). Panels below show the differential reactivity of HC and HS3 sera pools against LZ after depletion on P. uenonis or P. bivia , expressed as AUC values from ELISA with serially diluted sera, and as the mean percentage of IgG loss in bacteria-depleted pools ( n = 3) compared to non-depleted sera, analyzed by two-way ANOVA with Sidak’s multiple comparisons test. .
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1144 pseudomonas aeruginosa atcc 27853 salmonella typhimurium bacillus subtilis atcc 6633 enterococcus faecalis atcc 29212 streptococcus  (ATCC)
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( A ) IgG (left) and IgA (right) reactivities of HC and HS3 serum pools towards healthy skin sections are shown. Scale bar: 100 µm. The white dotted line indicates the separation between epidermis (Epi) and dermis. ( B ) Same as in ( A ) towards healthy donor-derived KCs, with quantification of IgG signals normalized to Dapi. Scale bar: 50 µm. Mann–Whitney Statistical test, n = 3. ( C ) Dot plots illustrating the reactivity of HC and HS3 sera ( n = 11) against <t>peptidoglycan</t> (PGN), Keyhole limpet hemocyanin (KLH), MAPK14, lipopolysaccharide (LPS), double-stranded DNA (dsDNA), tubulin (TB), and lysozyme (LZ). Data were area under the curve (AUC) values determined by ELISA with serially diluted sera. Mann–Whitney statistical test. ( D ) Methodology for depleting HC and HS3 serum pools on bacteria (top). Panels below show the differential reactivity of HC and HS3 sera pools against LZ after depletion on P. uenonis or P. bivia , expressed as AUC values from ELISA with serially diluted sera, and as the mean percentage of IgG loss in bacteria-depleted pools ( n = 3) compared to non-depleted sera, analyzed by two-way ANOVA with Sidak’s multiple comparisons test. .
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( A ) IgG (left) and IgA (right) reactivities of HC and HS3 serum pools towards healthy skin sections are shown. Scale bar: 100 µm. The white dotted line indicates the separation between epidermis (Epi) and dermis. ( B ) Same as in ( A ) towards healthy donor-derived KCs, with quantification of IgG signals normalized to Dapi. Scale bar: 50 µm. Mann–Whitney Statistical test, n = 3. ( C ) Dot plots illustrating the reactivity of HC and HS3 sera ( n = 11) against <t>peptidoglycan</t> (PGN), Keyhole limpet hemocyanin (KLH), MAPK14, lipopolysaccharide (LPS), double-stranded DNA (dsDNA), tubulin (TB), and lysozyme (LZ). Data were area under the curve (AUC) values determined by ELISA with serially diluted sera. Mann–Whitney statistical test. ( D ) Methodology for depleting HC and HS3 serum pools on bacteria (top). Panels below show the differential reactivity of HC and HS3 sera pools against LZ after depletion on P. uenonis or P. bivia , expressed as AUC values from ELISA with serially diluted sera, and as the mean percentage of IgG loss in bacteria-depleted pools ( n = 3) compared to non-depleted sera, analyzed by two-way ANOVA with Sidak’s multiple comparisons test. .
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( A ) IgG (left) and IgA (right) reactivities of HC and HS3 serum pools towards healthy skin sections are shown. Scale bar: 100 µm. The white dotted line indicates the separation between epidermis (Epi) and dermis. ( B ) Same as in ( A ) towards healthy donor-derived KCs, with quantification of IgG signals normalized to Dapi. Scale bar: 50 µm. Mann–Whitney Statistical test, n = 3. ( C ) Dot plots illustrating the reactivity of HC and HS3 sera ( n = 11) against <t>peptidoglycan</t> (PGN), Keyhole limpet hemocyanin (KLH), MAPK14, lipopolysaccharide (LPS), double-stranded DNA (dsDNA), tubulin (TB), and lysozyme (LZ). Data were area under the curve (AUC) values determined by ELISA with serially diluted sera. Mann–Whitney statistical test. ( D ) Methodology for depleting HC and HS3 serum pools on bacteria (top). Panels below show the differential reactivity of HC and HS3 sera pools against LZ after depletion on P. uenonis or P. bivia , expressed as AUC values from ELISA with serially diluted sera, and as the mean percentage of IgG loss in bacteria-depleted pools ( n = 3) compared to non-depleted sera, analyzed by two-way ANOVA with Sidak’s multiple comparisons test. .
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Image Search Results


( A ) IgG (left) and IgA (right) reactivities of HC and HS3 serum pools towards healthy skin sections are shown. Scale bar: 100 µm. The white dotted line indicates the separation between epidermis (Epi) and dermis. ( B ) Same as in ( A ) towards healthy donor-derived KCs, with quantification of IgG signals normalized to Dapi. Scale bar: 50 µm. Mann–Whitney Statistical test, n = 3. ( C ) Dot plots illustrating the reactivity of HC and HS3 sera ( n = 11) against peptidoglycan (PGN), Keyhole limpet hemocyanin (KLH), MAPK14, lipopolysaccharide (LPS), double-stranded DNA (dsDNA), tubulin (TB), and lysozyme (LZ). Data were area under the curve (AUC) values determined by ELISA with serially diluted sera. Mann–Whitney statistical test. ( D ) Methodology for depleting HC and HS3 serum pools on bacteria (top). Panels below show the differential reactivity of HC and HS3 sera pools against LZ after depletion on P. uenonis or P. bivia , expressed as AUC values from ELISA with serially diluted sera, and as the mean percentage of IgG loss in bacteria-depleted pools ( n = 3) compared to non-depleted sera, analyzed by two-way ANOVA with Sidak’s multiple comparisons test. .

Journal: EMBO Molecular Medicine

Article Title: A skin colonizer disrupts inflammatory and humoral immune defenses in hidradenitis suppurativa

doi: 10.1038/s44321-026-00407-7

Figure Lengend Snippet: ( A ) IgG (left) and IgA (right) reactivities of HC and HS3 serum pools towards healthy skin sections are shown. Scale bar: 100 µm. The white dotted line indicates the separation between epidermis (Epi) and dermis. ( B ) Same as in ( A ) towards healthy donor-derived KCs, with quantification of IgG signals normalized to Dapi. Scale bar: 50 µm. Mann–Whitney Statistical test, n = 3. ( C ) Dot plots illustrating the reactivity of HC and HS3 sera ( n = 11) against peptidoglycan (PGN), Keyhole limpet hemocyanin (KLH), MAPK14, lipopolysaccharide (LPS), double-stranded DNA (dsDNA), tubulin (TB), and lysozyme (LZ). Data were area under the curve (AUC) values determined by ELISA with serially diluted sera. Mann–Whitney statistical test. ( D ) Methodology for depleting HC and HS3 serum pools on bacteria (top). Panels below show the differential reactivity of HC and HS3 sera pools against LZ after depletion on P. uenonis or P. bivia , expressed as AUC values from ELISA with serially diluted sera, and as the mean percentage of IgG loss in bacteria-depleted pools ( n = 3) compared to non-depleted sera, analyzed by two-way ANOVA with Sidak’s multiple comparisons test. .

Article Snippet: Peptidoglycan from B. subtilis , Invivogen , Cat# tlrl-pgnb3.

Techniques: Derivative Assay, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay, Bacteria