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Cell viability by MTT assay to determine the effects of live and UV-killed <t>B.</t> <t>subtilis</t> <t>natto</t> on Caco-2 cells. Different MOIs (MOI 1, 10, 100) of live and UV-killed B. subtilis natto were added to Caco-2 cell monolayer and incubated for 24 h. The data are expressed as the mean ± SEM of three independent experiments. The results are presented as the percentage of cell survival compared to control samples. MOI, multiplicity of infection; Bsn, B. subtilis natto.
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Cell viability by MTT assay to determine the effects of live and UV-killed B. subtilis natto on Caco-2 cells. Different MOIs (MOI 1, 10, 100) of live and UV-killed B. subtilis natto were added to Caco-2 cell monolayer and incubated for 24 h. The data are expressed as the mean ± SEM of three independent experiments. The results are presented as the percentage of cell survival compared to control samples. MOI, multiplicity of infection; Bsn, B. subtilis natto.

Journal: Iranian Journal of Microbiology

Article Title: Immunomodulatory effects of live and UV-killed Bacillus subtilis natto on inflammatory response in human colorectal adenocarcinoma cell line in vitro

doi: 10.18502/ijm.v16i4.16301

Figure Lengend Snippet: Cell viability by MTT assay to determine the effects of live and UV-killed B. subtilis natto on Caco-2 cells. Different MOIs (MOI 1, 10, 100) of live and UV-killed B. subtilis natto were added to Caco-2 cell monolayer and incubated for 24 h. The data are expressed as the mean ± SEM of three independent experiments. The results are presented as the percentage of cell survival compared to control samples. MOI, multiplicity of infection; Bsn, B. subtilis natto.

Article Snippet: B. subtilis natto (ATCC 7059) was obtained from World Intellectual Resource Co. (Taiwan).

Techniques: MTT Assay, Incubation, Control, Infection

Effects of live (MOI 100) and UV-killed (MOI 100) B. subtilis natto (Bsn) on the expression levels of anti-inflammatory genes IL-10 (A) and TGF-β (B) in Caco-2 cells by RT-qPCR. Expression data are normalized using β-actin as a reference gene. Caco-2 cells monolayers were treated with LPS for 24 h as a positive control. Data are shown as mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001 compared to the untreated control were considered statistically significant.

Journal: Iranian Journal of Microbiology

Article Title: Immunomodulatory effects of live and UV-killed Bacillus subtilis natto on inflammatory response in human colorectal adenocarcinoma cell line in vitro

doi: 10.18502/ijm.v16i4.16301

Figure Lengend Snippet: Effects of live (MOI 100) and UV-killed (MOI 100) B. subtilis natto (Bsn) on the expression levels of anti-inflammatory genes IL-10 (A) and TGF-β (B) in Caco-2 cells by RT-qPCR. Expression data are normalized using β-actin as a reference gene. Caco-2 cells monolayers were treated with LPS for 24 h as a positive control. Data are shown as mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001 compared to the untreated control were considered statistically significant.

Article Snippet: B. subtilis natto (ATCC 7059) was obtained from World Intellectual Resource Co. (Taiwan).

Techniques: Expressing, Quantitative RT-PCR, Positive Control, Control

Effects of live (MOI 100) and UV-killed (MOI 100) B. subtilis natto (Bsn) on TLR2 (A) and TLR4 (B) gene expression in Caco-2 cells after 24 h. Gene expression data are normalized using β-actin as the reference gene. Caco-2 cell monolayers were treated with LPS for 24 h as a positive control. Data are presented as the mean ± SEM of three independent experiments. *P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001 was considered statistically significant compared to the untreated control.

Journal: Iranian Journal of Microbiology

Article Title: Immunomodulatory effects of live and UV-killed Bacillus subtilis natto on inflammatory response in human colorectal adenocarcinoma cell line in vitro

doi: 10.18502/ijm.v16i4.16301

Figure Lengend Snippet: Effects of live (MOI 100) and UV-killed (MOI 100) B. subtilis natto (Bsn) on TLR2 (A) and TLR4 (B) gene expression in Caco-2 cells after 24 h. Gene expression data are normalized using β-actin as the reference gene. Caco-2 cell monolayers were treated with LPS for 24 h as a positive control. Data are presented as the mean ± SEM of three independent experiments. *P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001 was considered statistically significant compared to the untreated control.

Article Snippet: B. subtilis natto (ATCC 7059) was obtained from World Intellectual Resource Co. (Taiwan).

Techniques: Gene Expression, Positive Control, Control