Journal: The Journal of Physiology

Article Title: Firing properties of single axons with cardiac rhythmicity in the human cervical vagus nerve

doi: 10.1113/JP286423

Figure Lengend Snippet: A summary of the characteristics of participants from whom cardiac rhythmic single‐units were successfully isolated

Article Snippet: A large proportion of baroreceptive neurones with unmyelinated axons are active at normal pressures in the anaesthetised rabbit (Thoren & Jones, ; Yao & Thoren, ).

Techniques: Isolation

Journal: The Journal of Physiology

Article Title: Firing properties of single axons with cardiac rhythmicity in the human cervical vagus nerve

doi: 10.1113/JP286423

Figure Lengend Snippet: Each panel depicts the overlain isolated spikes, cardiac cross‐correlograms, and respiratory cross‐correlograms of an axon recorded during slow, deep breathing in three different participants, as well as the corresponding average of respiratory belt movement (mean [solid black line] ± standard deviation [dotted black line]). A , participant 1, right vagus; B , participant 4, left vagus; C , participant 7, right vagus. Neurones were designated as putative cardiac efferents if they had negative‐going spikes (indicative of unmyelinated axons), displayed cardiac rhythmicity on their corresponding cardiac cross‐correlogram, and post‐inspiratory or expiratory rhythmicity on their corresponding respiratory cross‐correlogram. Rhythmicity was defined by the presence of evident cardiac phasic or respiratory phasic fluctuations in the identified spike's firing frequency. Three such neurones were identified: two from the right vagus and one from the left. I‐PI, inspiratory‐post‐inspiratory transition

Article Snippet: A large proportion of baroreceptive neurones with unmyelinated axons are active at normal pressures in the anaesthetised rabbit (Thoren & Jones, ; Yao & Thoren, ).

Techniques: Isolation, Standard Deviation

Journal: Nature communications

Article Title: A mesocortical glutamatergic pathway modulates neuropathic pain independent of dopamine co-release.

doi: 10.1038/s41467-024-45035-2

Figure Lengend Snippet: Fig. 2 | Optogenetic activation of VTA glutamatergic terminals in the PL alle- viates pain-associated behaviors. a Experimental design to express hChR2 or mCherry (control) in VTA glutamatergic neurons and stimulate their terminals in the PL. b Schematic of real-time place preference (RT-PP) test for assessing ongoing pain. c Preference to stay in the light stimulation-paired chamber for SNI mice expressing mCherry or hChR2 and sham mice expressing hChR2 (P = 0.0036 and 0.0137 for SNI+hChR2 vs. SNI+mCherry and sham+hChR2, respectively). d Schematic of conditioned place preference (CPP) test for assessing ongoing pain. e Preference to stay in the light stimulation-paired chamber for SNI mice expressing mCherry or hChR2 and sham mice expressing hChR2 (P = 0.0077 and 0.0061 for

Article Snippet: To image Ca2+ in the axons of VTA glutamatergic neurons, 0.2 μl of AAV5-hSynapsin1-FLEx-axon-GCaMP6s (112010; Addgene) was slowly injected into the VTA (AP −3.28mm,ML 0.36mm, subpial (SP) 4.13mm)ofVglut2IRES-Cremiceover 15min using a picospritzer (15 p.s.i., 10ms pulse width, 0.5 Hz) via a glass microelectrode.

Techniques: Activation Assay, Control, Expressing, Conditioned Place Preference