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axioimager m2 fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axioimager m2 fluorescence microscope
    Axioimager M2 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioimager+m2+microscope/software+zen/bio_rxiv__2025__07__11__663895-203-8-7
    Average 90 stars, based on 1 article reviews
    axioimager m2 fluorescence microscope - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Cell-type specific sensory and motor activity in the cuneiform nucleus and pedunculopontine nucleus in mice.
    Article Snippet: .. Brain sections were observed using a Zeiss AxioImager M2 microscope bundled with StereoInvestigator 2018 software (v1.1, MBF Bioscience). ..

    Article Title: Methionine cycle in C. elegans serotonergic neurons regulates diet-dependent behaviour and longevity through neuron-gut signaling.
    Article Snippet: .. The GFP fluorescence emitted by the worms was captured using an AxioImager M2 microscope (Carl Zeiss, Germany) at a magnification of 10X. ..

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma.
    Article Snippet: All cells were then incubated with the secondary antibody Alexa Fluor 488 Phalloidin (Applied Biosystems ThermoFisher, Pleasanton, CA) at Scientific Reports | (2025) 15:23044 15| https://doi.org/10.1038/s41598-025-05517-9 a 1:500 dilution. .. Fluorescent images were obtained using a Zeiss Axioimager M2 microscope equipped with an Axiocam 503 imaging device and Zeiss ZEN software (Zeiss Microscopy, 40 × magnification). .. Paraffin-embedded brain tumour tissue array slides (US Biomax Inc., London, UK) were acquired following Health Insurance Portability and Accountability Act (HIPAA) approved protocols to ensure ethical standards.

    Article Title: TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function
    Article Snippet: .. After incubation, the cells were washed twice with Incubation Buffer and imaged using a Zeiss AxioImager M2 microscope equipped with dual-wavelength settings: JC-1 aggregates (indicative of intact ΔΨm, red fluorescence: Ex 488 nm/Em 590–650 nm) and monomers (indicative of depolarized ΔΨm, green fluorescence: Ex 488 nm/Em 500–550 nm). ..

    Article Title: Nutritionally Responsive <scp>PMv DAT</scp> Neurons Are Dynamically Regulated During Pubertal Transition
    Article Snippet: After all incubation steps following the kit's protocol, slides were incubated in DAPI solution for 30 s at room temperature and coverslipped using ProLong Gold Antifade Mountant (ThermoFisher Scientific). .. Quantification of mRNA coexpression within cells was performed on PMv images acquired with a 40× oil objective on an AxioImager M2 microscope (Zeiss). ..

    Article Title: RICTOR regulates an interspecies crosstalk that influences longevity through a novel methionine cycle-mitophagy axis
    Article Snippet: .. Fluorescent microscopy was carried out using an AxioImager M2 microscope (Carl Zeiss, Germany) at a magnification of 10X. ..

    Software:

    Article Title: Cell-type specific sensory and motor activity in the cuneiform nucleus and pedunculopontine nucleus in mice.
    Article Snippet: .. Brain sections were observed using a Zeiss AxioImager M2 microscope bundled with StereoInvestigator 2018 software (v1.1, MBF Bioscience). ..

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma.
    Article Snippet: All cells were then incubated with the secondary antibody Alexa Fluor 488 Phalloidin (Applied Biosystems ThermoFisher, Pleasanton, CA) at Scientific Reports | (2025) 15:23044 15| https://doi.org/10.1038/s41598-025-05517-9 a 1:500 dilution. .. Fluorescent images were obtained using a Zeiss Axioimager M2 microscope equipped with an Axiocam 503 imaging device and Zeiss ZEN software (Zeiss Microscopy, 40 × magnification). .. Paraffin-embedded brain tumour tissue array slides (US Biomax Inc., London, UK) were acquired following Health Insurance Portability and Accountability Act (HIPAA) approved protocols to ensure ethical standards.

    Fluorescence:

    Article Title: Methionine cycle in C. elegans serotonergic neurons regulates diet-dependent behaviour and longevity through neuron-gut signaling.
    Article Snippet: .. The GFP fluorescence emitted by the worms was captured using an AxioImager M2 microscope (Carl Zeiss, Germany) at a magnification of 10X. ..

    Article Title: TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function
    Article Snippet: .. After incubation, the cells were washed twice with Incubation Buffer and imaged using a Zeiss AxioImager M2 microscope equipped with dual-wavelength settings: JC-1 aggregates (indicative of intact ΔΨm, red fluorescence: Ex 488 nm/Em 590–650 nm) and monomers (indicative of depolarized ΔΨm, green fluorescence: Ex 488 nm/Em 500–550 nm). ..

    Imaging:

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma.
    Article Snippet: All cells were then incubated with the secondary antibody Alexa Fluor 488 Phalloidin (Applied Biosystems ThermoFisher, Pleasanton, CA) at Scientific Reports | (2025) 15:23044 15| https://doi.org/10.1038/s41598-025-05517-9 a 1:500 dilution. .. Fluorescent images were obtained using a Zeiss Axioimager M2 microscope equipped with an Axiocam 503 imaging device and Zeiss ZEN software (Zeiss Microscopy, 40 × magnification). .. Paraffin-embedded brain tumour tissue array slides (US Biomax Inc., London, UK) were acquired following Health Insurance Portability and Accountability Act (HIPAA) approved protocols to ensure ethical standards.

    Incubation:

    Article Title: TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function
    Article Snippet: .. After incubation, the cells were washed twice with Incubation Buffer and imaged using a Zeiss AxioImager M2 microscope equipped with dual-wavelength settings: JC-1 aggregates (indicative of intact ΔΨm, red fluorescence: Ex 488 nm/Em 590–650 nm) and monomers (indicative of depolarized ΔΨm, green fluorescence: Ex 488 nm/Em 500–550 nm). ..

    other:

    Article Title:
    Article Snippet: Images of H&E-28 stained cervical tumors and epithelia were captured using a Zeiss AxioImager M2 29 microscope and AxioVision software version 4.8.2 (Jena, Germany).

    Article Title: A type IV pili-mediated mutualism between two co-resident temperate archaeal viruses and their host.
    Article Snippet: Images were acquired using an Olympus BX53 upright microscope with a Retiga R1 camera from QImaging, a CoolLED pE-4000 light source and a U-Plan X Apochromat phase contrast objective lens (100×, 1.45 numerical aperture [NA], oil immersion).



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    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
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    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
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    Carl Zeiss axioimager m2 epifluorescence microscope
    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
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    Image Search Results


    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under fluorescence microscope show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.

    Journal: Clinical Ophthalmology (Auckland, N.Z.)

    Article Title: TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function

    doi: 10.2147/OPTH.S521660

    Figure Lengend Snippet: HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under fluorescence microscope show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.

    Article Snippet: Fluorescence images were captured using a Zeiss AxioImager M2 fluorescence microscope (Zeiss, Germany) with the following parameters: Oxidized state (green fluorescence): Excitation wavelength of 488 nm, emission filter of 500–550 nm;- Reduced state (red fluorescence): Excitation wavelength of 581 nm, emission filter of 590–650 nm.Images were analyzed using Zen 3.1 software and quantified with ImageJ software.

    Techniques: Cell Culture, Staining, Fluorescence, Microscopy, Polymer, Control, Western Blot, Extraction, Quantitative RT-PCR, Expressing