Journal: BMC Musculoskeletal Disorders

Article Title: Human telomerase reverse transcriptase and glucose-regulated protein 78 increase the life span of articular chondrocytes and their repair potential

doi: 10.1186/1471-2474-13-51

Figure Lengend Snippet: Macroscopic images of the cell-scaffold complex after 14 days of culture . Atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS) scaffold complex seeded with nontransfected (A), GRP78 -transfected (B), hTERT -transfected (C), or hTERT - and GRP78 -transfected (D) ORA chondrocytes. Scale bar = 1.0 mm.

Article Snippet: Briefly, nontransfected and hTERT / GRP78 -transfected ORA chondrocytes were passaged twice, and then seeded at a density of 2 × 10 6 cells·scaffold -1 into a round ACHMS scaffold (diameter, 6 mm; thickness, 2 mm; average pore size, 200 μm) [ , , , ] in 48-well plates (Sumitomo Bakelite, Tokyo, Japan) by centrifuging at 45 g for 5 min. Then, these cell-seeded scaffolds were cultured in BM supplemented with 10% FBS at 37°C in an incubator with 5% CO 2 and 100% relative humidity for 14 d. These cultured chondrocytes were frozen in liquid nitrogen until needed for biochemical analyses and transplantation into an in vivo model of articular cartilage defects.

Techniques: Transfection