Journal: JAMA Network Open

Article Title: Costs and Causes of Oncology Drug Attrition With the Example of Insulin-Like Growth Factor-1 Receptor Inhibitors

doi: 10.1001/jamanetworkopen.2023.24977

Figure Lengend Snippet: The 16 IGF-1R Inhibitors and the Estimated Number of Patients Entered Into Clinical Trials ^a

Article Snippet: AVE1642 , Antibody , Sanofi-Aventis , 57.

Techniques: Clinical Proteomics

Journal: JAMA Network Open

Article Title: Costs and Causes of Oncology Drug Attrition With the Example of Insulin-Like Growth Factor-1 Receptor Inhibitors

doi: 10.1001/jamanetworkopen.2023.24977

Figure Lengend Snippet: Activity of Single Agent IGF-1R Inhibitors on a Selection of Tumor Xenograft Models

Article Snippet: AVE1642 , Antibody , Sanofi-Aventis , 57.

Techniques: Activity Assay, Selection, Inhibition

Journal: Frontiers in Immunology

Article Title: Monoclonal Antibody Therapies for Hematological Malignancies: Not Just Lineage-Specific Targets

doi: 10.3389/fimmu.2017.01936

Figure Lengend Snippet: Characteristics of antibodies directed to NLSAs.

Article Snippet: , AVE1642 (ImmunoGen) , Hz IgG1 , Blocks IGF-1R , I (MM/NCT01233895 C) disc. .

Techniques:

Journal: Oncotarget

Article Title: CD99 triggering induces methuosis of Ewing sarcoma cells through IGF-1R/RAS/Rac1 signaling

doi: 10.18632/oncotarget.13160

Figure Lengend Snippet: A. CD99-induced cell death in parental or IGF-1R silenced TC-71 cells (Annexin V/PI) (upper panel). Results are represented as mean ± SEM of three independent experiments (*p<0.05, Student's t test). Western blotting evaluation of pan-RAS levels before (−) and after (+) treatment with 0662mAb in corresponding cells (lower panel). B. Vacuolization was assessed by Acridine Orange staining (AO) after 0662mAb treatment in parental (TC-71) or IGF-1R silenced cells (TC-siIGF1R#59) (scale bar 20μm). C. Experiments were performed in TC-71 cells before (−) and after (+) 15 min treatment with 0662mAb alone or in presence of either hAb AVE1642 or NVP-AEW541 (Annexin V/PI). Histograms (upper left panel) represent mean percentage ± SEM of dead cells (*p<0.05, Student's t test); western blotting (upper right panel) evaluates pan-RAS levels in the same conditions. Lower panels display AO staining before (CTR) and after 3h treatment with 0662mAb alone or in presence of either hAb AVE1642 or NVP-AEW541. For AO staining, cells were acquired using the microscope Nikon ECLIPSE 90i with Plan Fluor 40x/0.75 DIC M/N2. Pictures provided in the figures are all merged images.

Article Snippet: The following inhibitors were used alone or in combination with 0662mAb: necrostatin-1 (50μM), 3-methyladenine (3-MA) (5mM, Sigma), Cytochalasin D (5μg/ml, Sigma) pre-treatment at 37°C for 60 min, chlorpromazine (10μg/ml, Sigma) pretreatment at 37°C for 30 min, anti-IGF-1R neutralizing antibody AVE1642 [ ] (100ng/ml, kindly provided by ImmunoGen Inc), tyrosine kinase inhibitor NVP-AEW541 (1μM, kindly provided by Novartis) pre-treatment at 37°C for 60 min.

Techniques: Western Blot, Staining, Microscopy