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automated image analysis algorithm color deconvolution  (Aperio Technologies)
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Aperio Technologies automated image analysis algorithm color deconvolution
Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color <t>deconvolution</t> (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.
Automated Image Analysis Algorithm Color Deconvolution, supplied by Aperio Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
automated image analysis algorithm color deconvolution - by Bioz Stars, 2026-08
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Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color deconvolution (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.

Journal: Cancers

Article Title: Lysyl-Oxidase Dependent Extracellular Matrix Stiffness in Hodgkin Lymphomas: Mechanical and Topographical Evidence

doi: 10.3390/cancers14010259

Figure Lengend Snippet: Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color deconvolution (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.

Article Snippet: Layers of each virtual slide annotation were created manually, selecting the whole area, and analyzed with the automated image analysis algorithm color deconvolution (Aperio software version 9.1, Aperio Technologies, Nussloch GmbH, Wetzlar, Germany).

Techniques: Imaging, Staining, Software