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Developmental patterns of 1-year-old P. ginseng storage roots. (A, B) Representative images of stained root cross-section of P. ginseng plants from 0 to 16 WAD, visualized using polarized light microscopy after staining with Safranin-O and Astra Blue. XV: xylem vessel, CZ: cambial cell layer zone, PH, phloem cells, RD: resin duct cells. Scale bar = (A) 100 μm, (B) 200 μm. (C) The numbers of cambial stem cells in the cambial cell layer zone (CZ) (n = 60).

Journal: Journal of Ginseng Research

Article Title: First-year seasonal growth regulation mechanism underlying storage root thickening in perennial Panax ginseng

doi: 10.1016/j.jgr.2025.09.002

Figure Lengend Snippet: Developmental patterns of 1-year-old P. ginseng storage roots. (A, B) Representative images of stained root cross-section of P. ginseng plants from 0 to 16 WAD, visualized using polarized light microscopy after staining with Safranin-O and Astra Blue. XV: xylem vessel, CZ: cambial cell layer zone, PH, phloem cells, RD: resin duct cells. Scale bar = (A) 100 μm, (B) 200 μm. (C) The numbers of cambial stem cells in the cambial cell layer zone (CZ) (n = 60).

Article Snippet: After dewaxing in HistoClear, the slides were rehydrated and counterstained with 1 % Safranin-O (Sigma, cat. S2255) and 0.8 % Astra Blue (Santa Cruz Biochem, cat. sc-214558A) for 3 min. Safranin-O preferentially stains lignified cell walls (e.g., xylem vessels) red, while Astra Blue stains cellulose-rich tissues blue, enabling the distinction of vascular and storage tissues.

Techniques: Staining, Light Microscopy