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ONERA The French Aerospace Lab
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HeiTel Digital Video
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Astra Pharmaceuticals AB
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Image Search Results
Journal:
Article Title: Synthesis of Biodegradable Multiblock Copolymers by Click Coupling of RAFT-Generated HeterotelechelicPolyHPMA Conjugates
doi: 10.1016/j.reactfunctpolym.2010.10.005
Figure Lengend Snippet: RAFT polymerization of HPMA mediated by CTA-GFLG-alkyne. A) Pseudo first- order rate plots for polymerizations in methanol at 60 °C and in water at 70 °C. B) Evolution of molecular weight (RI detection; Superose 12 column) with time for HPMA polymerization in methanol at 60 °C. C) Plot of Mn versus conversion for HPMA polymerization, D) SEC chromatogram (RI detection; Superose 6 column) of RAFT polymerization of HPMA in water at 70 °C using polyHPMA as macro-CTA, [M]0/[macro-CTA] = 500, Mth = 66700 (ρ=70%) and showing the evolution of molecular weight.
Article Snippet: Open in a separate window Figure 5 A) SEC
Techniques: Molecular Weight
Journal:
Article Title: Synthesis of Biodegradable Multiblock Copolymers by Click Coupling of RAFT-Generated HeterotelechelicPolyHPMA Conjugates
doi: 10.1016/j.reactfunctpolym.2010.10.005
Figure Lengend Snippet: A) SEC chromatogram (RI detection; Superose 6 column) of initial P-gemcitabine conjugate (dashed line) and the multiblock copolymer after chain extension (full line); B) SEC chromatogram (RI detection; Superose 6 column) of the multiblock copolymer after chain extension after Astra software multipeak fitting. C) Chain extension of heterotelechelic copolymer of HPMA with MA-GFLG-gemcitabine (Mw = 28 kDa, Mw/Mn = 1.06) by copper catalyzed alkyne and azide 1,3-dipolar cycloaddition into a multi block copolymer. (selected fraction of Mw = 224 kDa, Mw/Mn = 1.09). Incubation of the multiblock copolymer (selected fraction; Mw = 224 kDa, Mw/Mn = 1.09) with 0.14 mg/mLpapain in citrate-phosphate buffer (pH 6.0) at 37°C resulted in total degradation of the oligopeptide sequences in multiblock copolymer backbone and formation of the polymer with close to initial molecular weight (Mw = 33 kDa, Mw/Mn = 1.04). D) HPLC traces of a) multiblock high molecular weight copolymer of HPMA-gemcitabine conjugate (P-gem, fraction of Mw = 224 kDa, Mw/Mn = 1.09; b) gemcitabine cleaved from multiblock high molecular weight copolymer (P-gem); c) gemcitabine cleaved from monomer, MA-GFLG-gemcitabine, and d) free gemcitabine,
Article Snippet: Open in a separate window Figure 5 A) SEC
Techniques: Software, Blocking Assay, Incubation, Polymer, Molecular Weight, High Molecular Weight