Journal: Biological chemistry

Article Title: The redox status of cysteine thiol residues of apolipoprotein E impacts on its lipid interactions.

doi: 10.1515/hsz-2019-0414

Figure Lengend Snippet: Figure 6: Influence of apoAII on the DMPC clearance activity of apoE. Recombinant apoE2, apoE3 and apoE4 (10 mg/l) were treated with TCEP at a final concentration of 2.5 mm for 30 min at 25°C and were subsequently incubated with recombinant apoAII at final concentrations of 0 (control-2), 30 and 300 mg/l for 24 h at 37°C followed by the clearance assay. Control-1 was the apoE isoforms treated only with TCEP. Turbidity values were normalized at time zero. Time-courses for DMPC mLV clearance by apoE2 (A), apoE3 (B) and apoE4 (C) were expressed using mean values from three independent experiments. The results of the statistical analysis are summarized in Supplementary Figure 6.

Article Snippet: Recombinant apoE isoforms (apoE2, apoE3 and apoE4) were purchased from BioVision, Inc. (Milpitas, CA, USA), and recombinant apoAII was supplied by Athens Research & Technology, Inc. (Athens, GA, USA).

Techniques: Activity Assay, Recombinant, Concentration Assay, Incubation, Control

Journal: Biological chemistry

Article Title: The redox status of cysteine thiol residues of apolipoprotein E impacts on its lipid interactions.

doi: 10.1515/hsz-2019-0414

Figure Lengend Snippet: Figure 7: Influence of apoAII on the formation of apoE-DMPC liposome complexes. ApoE-DMPC liposome complexes, prepared as described in the Materials and methods section, were separated by non-denaturing PAGE using a 4–12% gradient gel, followed by detection with anti-apoE and anti-apoAII antibodies. The Stokes diameters of the complexes were determined from a calibration curve, which was constructed by plotting mobility against the corresponding Stokes diameters of a commercial size marker.

Article Snippet: Recombinant apoE isoforms (apoE2, apoE3 and apoE4) were purchased from BioVision, Inc. (Milpitas, CA, USA), and recombinant apoAII was supplied by Athens Research & Technology, Inc. (Athens, GA, USA).

Techniques: Construct, Marker