anti-kir4.1 (kcnj10) antibody  (Alomone Labs)

Journal: Journal of Sport and Health Science

Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

doi: 10.1016/j.jshs.2025.101111

Figure Lengend Snippet: Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

Techniques: Staining, Membrane

Journal: Journal of Sport and Health Science

Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

doi: 10.1016/j.jshs.2025.101111

Figure Lengend Snippet: HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

Techniques: Staining, Membrane, Comparison

Journal: Journal of Sport and Health Science

Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

doi: 10.1016/j.jshs.2025.101111

Figure Lengend Snippet: HSP abundances in type I and II skeletal fibers from older adults pre- and post HIT exercise. Relative protein abundances of (A and B) HSP72, (C and D) HSP27, (E and F) pHSP27 Ser15, (G and H) αB-crystallin, and (I and J) pαB-crystallin Ser59 in fibers from old pre and old post HIT exercise. All fibers are expressed relative to the average old pre type I fibers or relative pre type II depending on fiber type. The same color is assigned to the same Individual, consistent in both graphs and all figures. * p < 0.05 and ** p < 0.01 indicated significant difference in paired t -test (except pHSP27 Ser15 Wilcoxon match-pair rank test). Representative blots are shown in . HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

Techniques:

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Elabela (ELA) or apelin treatment did not alter sFlt-1 variants in CoCl 2 -treated HTR- 8/SVneo cells. Data are mean ± SEM, n=3-6.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques:

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: pSTAT3/STAT3 ratio and SOCS3 protein levels in response to apelin (A; Panels A and B) or elabela (ELA; Panels C and D) in CoCl 2 -treated HTR-8/SVneo Cells. Data are mean ± SEM, n=5-9.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques:

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin ELISA assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Elabela (ELA) or apelin treatment decreased IL-6 mRNA in CoCl 2 -treated HTR-8/SVneo cells. Data are mean ± SEM, n=3-6.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques:

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Soluble and membrane-bound gp130 in response to apelin (A; Panels A-B) or elabela (ELA; Panels C-D) in CoCl 2 -treated HTR-8/SVneo cells. Data are mean ± SEM, n=6 in each group.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques: Membrane

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Soluble and membrane-bound IL-6R in response to apelin (A; Panels A-B) or elabela (ELA; Panels C-D) in CoCl 2 -treated HTR-8/SVneo cells. Data are mean ± SEM, n=5-6.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques: Membrane

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Schematic of proposed interactions between IL-6 signaling and apelin and elabela in HTR-8/SVneo trophoblastic cells under CoCl₂-mimicked hypoxia. We hypothesize that apelin/elabela/APJ axis regulates IL-6 signaling via its actions on various components of the IL-6 classical and trans-signaling pathway.

Article Snippet: ELISA kits were used for apelin (RayBiotech Inc., Norcross, GA, USA, cat. No. EIA-APC) and elabela (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; cat. No. EK-0017-19) protein quantification in media and cell supernatant per manufacturer’s recommended protocol.

Techniques: