Journal: bioRxiv

Article Title: EctoShed : A novel Gene Delivery Platform for Functional Analysis of Adipocyte-Shed Proteoforms

doi: 10.1101/2025.10.14.682314

Figure Lengend Snippet: (a) Representative scheme illustrating the LV constructs utilized for overexpression of AOC3 proteoforms upon transduction of SVF-isolated primary white adipocytes (1° iWad) on day 4 of differentiation: full length Aoc3 ; soluble Aoc3 mimic (m- sAoc3) generated by fusing Adiponectin signal peptide to the proximal extracellular sequence of Aoc3 ; and control construct expressing Gfp , under a CMV promoter. Generated using Biorender. Relative mRNA expression levels of (b) Gfp , (c) Aoc3 , (d) m- sAoc3 in 1° iWad lysates. Fluorometric determination of AOC3 enzyme activity in (e) 1° iWad lysates and (f) 1° iWad supernatant. (g) Representative bright-field images of 1° iWad transduced with LV vectors (left); representative immunofluorescent (IF) images of 1° iWad transduced with LV vectors followed by DAPI and AOC3 labelling and (h) quantification of AOC3 in 1° iWad. Quantification of IF shows individual points for independent experiments. Statistics: bar graphs represent the mean + s.e.m. with all data point represented. Unpaired, two-tailed Student’s test were performed (b,c,d), Non-parametric, Kruskal-Wallis with Dunn’s post hoc correction were performed (e,f,h).

Article Snippet: AOC3 protein content in serum and iWAT lysates was quantified by ELISA (Novus Biologicals, NBP2-78769) according to the manufacturer’s protocol and normalized to total protein or sample volume.

Techniques: Construct, Over Expression, Transduction, Isolation, Generated, Sequencing, Control, Expressing, Activity Assay, Two Tailed Test

Journal: bioRxiv

Article Title: EctoShed : A novel Gene Delivery Platform for Functional Analysis of Adipocyte-Shed Proteoforms

doi: 10.1101/2025.10.14.682314

Figure Lengend Snippet: Representative scheme illustrating the AAV constructs utilized for overexpression of AOC3 proteoforms in iWAT upon cirurgical preparation: full length Aoc3 ; soluble Aoc3 mimic (m- sAoc3) generated by fusing Adiponectin signal peptide to the proximal extracellular sequence of Aoc3 ; and control construct expressing Gfp , under a miniUcp1 promoter. Generated using Biorender. Relative mRNA expression levels of (b) Gfp , (c) Aoc3, and (d) m- sAoc3 in iWAT. (e) Expression levels of indicated genes in iWAT, eWAT, liver and brown adipose tissue (BAT). (f) Fluorometric determination of AOC3 enzyme activity in iWAT and AOC3 protein quantification by enzyme-linked sorbent assay (ELISA) in (g) iWAT lysates and (h) serum. (i) Representative western blot showing GFP and AOC3 protein content in iWAT of transduced mice. HSC70 was used as loading control. Relative expression quantification is annotated in the figure. Statistics: bar graphs represent the mean + s.e.m. with all data point represented. Unpaired, two-tailed Student’s test were performed (b,c,d), Non-parametric, Kruskal-Wallis with Dunn’s post hoc correction were performed (e,f,g,h).

Article Snippet: AOC3 protein content in serum and iWAT lysates was quantified by ELISA (Novus Biologicals, NBP2-78769) according to the manufacturer’s protocol and normalized to total protein or sample volume.

Techniques: Construct, Over Expression, Generated, Sequencing, Control, Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Two Tailed Test

Journal: bioRxiv

Article Title: EctoShed : A novel Gene Delivery Platform for Functional Analysis of Adipocyte-Shed Proteoforms

doi: 10.1101/2025.10.14.682314

Figure Lengend Snippet: (a) Body weight (grams), (b) iWAT and (c) eWAT weight percentage relative to BW, and (d) fasted (6h) blood glucose levels 6 weeks after AAV-driven expression of m-s Aoc3 versus Gfp . Statistics: bar graphs represent the mean + s.e.m. with all data point represented. Non-parametric, two-tailed Mann-Whitney t-test was performed (a,b,c,d).

Article Snippet: AOC3 protein content in serum and iWAT lysates was quantified by ELISA (Novus Biologicals, NBP2-78769) according to the manufacturer’s protocol and normalized to total protein or sample volume.

Techniques: Expressing, Two Tailed Test, MANN-WHITNEY

Journal: Frontiers in Oncology

Article Title: Screening and identification of novel protein markers of early-stage lung cancer and construction and application of screening models

doi: 10.3389/fonc.2025.1567673

Figure Lengend Snippet: Survival analysis of the candidate protein markers. (A) The survival curve of CLEC3B. (B) The survival curve of AOC3. (C) The survival curve of HBB. (D) The survival curve of CAT. (E) The survival curve of SEPP1. (F) The survival curve of FGA. (G) The survival curve of ORM1. HR, hazard ratio; CLEC3B, C-type lectin domain family 3 member B; AOC3, membrane primary amine oxidase; HBB, hemoglobin subunit beta; CAT, catalase; SEPP1, selenoprotein P; FGA, Fibrinogen alpha chain; ORM1, Alpha-1-acid glycoprotein 1.

Article Snippet: The levels of AOC3, CAT, CLEC3B, SEPP1, and HBB in the plasma and alveolar lavage fluid of mice were measured using ELISA (Cusabio Biotechnology, Wuhan, China).

Techniques: Membrane

Journal: Frontiers in Oncology

Article Title: Screening and identification of novel protein markers of early-stage lung cancer and construction and application of screening models

doi: 10.3389/fonc.2025.1567673

Figure Lengend Snippet: Expression of candidate protein markers in cells of different passages. Expression and comparison of AOC3, CAT, CLEC3B, and SEPP1 among saline group, DMSO group and CTPE exposure group (A) in passage 10 (* CTPE vs . Saline group, P < 0.05). (B) in passage 20 (* CTPE vs . Saline group, P < 0.05). (C) in passage 30 (* CTPE vs . Saline group, P < 0.05). (D) in passage 40 (* CTPE vs . Saline group, P < 0.05). (E) Expression and comparison of AOC3, CAT, CLEC3B, and SEPP1 between BEAS-2B and A549 (*: A549 vs . BEAS-2B, P < 0.05).

Article Snippet: The levels of AOC3, CAT, CLEC3B, SEPP1, and HBB in the plasma and alveolar lavage fluid of mice were measured using ELISA (Cusabio Biotechnology, Wuhan, China).

Techniques: Expressing, Comparison, Saline

Journal: Frontiers in Oncology

Article Title: Screening and identification of novel protein markers of early-stage lung cancer and construction and application of screening models

doi: 10.3389/fonc.2025.1567673

Figure Lengend Snippet: Expression of candidate protein markers in lung tissue of mice in different months. Expressions and comparisons of AOC3, CAT, CLEC3B, SEPP1, and HBB among normal control (NC) group, vehicle control (VC) group and CTPE exposure group (A) in the 3rd month. (B) in the 6th month. (C) in the 9th month. (D) in the 12th month (* CTPE vs . NC, P < 0.05).

Article Snippet: The levels of AOC3, CAT, CLEC3B, SEPP1, and HBB in the plasma and alveolar lavage fluid of mice were measured using ELISA (Cusabio Biotechnology, Wuhan, China).

Techniques: Expressing, Control

Journal: Communications Biology

Article Title: Crosstalk of cell death pathways unveils an autophagy-related gene AOC3 as a critical prognostic marker in colorectal cancer

doi: 10.1038/s42003-024-05980-6

Figure Lengend Snippet: a Univariate Cox analysis of CDN4 feature genes with p < 0.05 and HR > 1 in TCGA and GEO prognosis cohorts. b Mean C-index of the candidate genes. c – f Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 group and low AOC3 group in TCGA and three validation datasets. g Representative immunohistochemistry (IHC) staining images of CRC tissue microarrays in low AOC3 tumor expression and high AOC3 tumor expression tissues. h Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 H-scores group and low AOC3 H-scores group in IHC arrays. i The molecules significantly associated with AOC3 were enriched in regulation of autophagy pathway. j Correlation analysis of AOC3 with regulation of autophagy pathways score. k Differential expression of autophagy genes in different AOC3 expressions. ns fdr>0.05, *fdr < 0.05, **fdr < 0.01, ***fdr < 0.001, ****fdr < 0.0001.

Article Snippet: Immunohistochemistry (IHC) was performed using AOC3 (1:600; Cat no. 66834-1-Ig, Proteintech, Wuhan, China).

Techniques: Biomarker Discovery, Immunohistochemistry, Expressing, Quantitative Proteomics