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Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of <t>cytokines</t> from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.
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Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of <t>cytokines</t> from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.
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Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of <t>cytokines</t> from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.
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Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of <t>cytokines</t> from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.
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Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of <t>cytokines</t> from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.
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Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of cytokines from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.

Journal: Journal of Extracellular Vesicles

Article Title: Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses

doi: 10.3402/jev.v4.28734

Figure Lengend Snippet: Flow cytometry strategy for intracellular cytokine production by spleen cells from infected mice. (a) Dot plots of CD4, CD8, CD19, F4/80/CD11b and MHCII/CD11c expression. The representative dot plots illustrating the frequency (%) of cytokines from spleen cells are shown for Colombiana and YuYu strains. (b) CD4+IL-10+ cells. (c) CD8+IL-10+. (d) CD19+TNF-α+. (e) MHC/CD11c+TNF-α+.

Article Snippet: Finally, cells were incubated in the dark (30 min) at room temperature, in the presence of 25 μL (1:50) of PE-labelled antimouse cytokines (IL-10, IFN-γ and TNF-α) (eBioscience).

Techniques: Flow Cytometry, Infection, Expressing

Intracellular cytokine production by T lymphocytes is similar after stimulation with EVs from Colombiana or YuYu strains. (a) CD4+ production of intracytoplasmic cytokines (IL-10 with IFN-γ and TNF-α). (b) CD8+ production of intracytoplasmic cytokines (IL-10 with IFN-γ and TNF-α). Immunophenotypic staining was performed as described in the Material and Methods section. Bars express the mean value±SD of 2 separate studies (*p<0.05).

Journal: Journal of Extracellular Vesicles

Article Title: Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses

doi: 10.3402/jev.v4.28734

Figure Lengend Snippet: Intracellular cytokine production by T lymphocytes is similar after stimulation with EVs from Colombiana or YuYu strains. (a) CD4+ production of intracytoplasmic cytokines (IL-10 with IFN-γ and TNF-α). (b) CD8+ production of intracytoplasmic cytokines (IL-10 with IFN-γ and TNF-α). Immunophenotypic staining was performed as described in the Material and Methods section. Bars express the mean value±SD of 2 separate studies (*p<0.05).

Article Snippet: Finally, cells were incubated in the dark (30 min) at room temperature, in the presence of 25 μL (1:50) of PE-labelled antimouse cytokines (IL-10, IFN-γ and TNF-α) (eBioscience).

Techniques: Staining