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Journal: Scientific Reports
Article Title: A novel loss-of-function mutation of PBK associated with human kidney stone disease
doi: 10.1038/s41598-020-66936-4
Figure Lengend Snippet: Downstream signaling hits between cells expressing wild-type PBK and cells expressing p.Gly43Arg PBK (control and treatment) for 24 hours as analyzed by KAM-900P Antibody Microarray.
Article Snippet: Protein phosphorylation in HEK293T cells transfected with plasmid construct containing either WT-PBK cDNA or mutant-p.G43R-PBK cDNA was examined by
Techniques: Expressing, Control, Microarray
Journal: International Journal of Molecular Medicine
Article Title: Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion
doi: 10.3892/ijmm.2016.2601
Figure Lengend Snippet: Kinexus antibody microarray. Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.
Article Snippet: The extracts were analyzed by the
Techniques: Microarray, Western Blot, Migration, Molecular Weight