Journal: PLOS Biology

Article Title: Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice

doi: 10.1371/journal.pbio.3003153

Figure Lengend Snippet: A. Volcano plot showing RNA-seq data of downregulated (purple open circles) and upregulated (purple filled circles) genes, using a 2-fold change (FC) and a p-value (< 0.05) as selection criteria, indicated by dashed lines. Bar plots showing significant fold changes (log2) in the expression profiles of transcripts encoding transcription factors (B) , homeobox and apoptosis-related proteins (C) , as well as ion channels, synaptic, and neurotransmission-related proteins (D) . Kcna3 : potassium voltage-gated channel, shaker-related subfamily, member 3. Kcnc3 : potassium voltage gated channel, Shaw-related subfamily, member 3. Kcne3 : potassium voltage-gated channel, Isk-related subfamily, gene 3. Kcnh3 : potassium voltage-gated channel, subfamily H (eag-related), member 3. Kcng4 : potassium voltage-gated channel, subfamily G, member 4. Kcnj12 : potassium inwardly-rectifying channel, subfamily J, member 12. Kcnk12 : potassium channel, subfamily K, member 12. Kctd12b : potassium channel tetramerization domain containing 12b. Hcn2 : hyperpolarization-activated, cyclic nucleotide-gated K + 2. Scn1b : sodium channel, voltage-gated, type I, beta. Piezo1 : piezo-type mechanosensitive ion channel component 1. Pkd2l1 : polycystic kidney disease 2-like 1. Trpv4 : transient receptor potential cation channel, subfamily V, member 4. Camkv : CaM kinase-like vesicle-associated. Sntb2 : syntrophin, basic 2. Syndig1l : synapse differentiation inducing 1 like. Rapsn : receptor-associated protein of the synapse. Grik5 : glutamate receptor, ionotropic, kainate 5 (gamma 2). Nos1ap : nitric oxide synthase 1 (neuronal) adaptor protein. Adra1d : adrenergic receptor, alpha 1d. Cckar : cholecystokinin A receptor. Sstr3 : somatostatin receptor 3. Trh : thyrotropin releasing hormone. Npy : neuropeptide Y. Gad2 : glutamic acid decarboxylase 2. Ascl2 : achaete-scute family bHLH transcription factor 2. Atf5 : activating transcription factor 5. Bex4 : brain expressed X-linked 4. Brf1 : BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit. Ddx4 : DEAD box helicase 4. E2f1 : E2F transcription factor 1. E2f8 : E2F transcription factor 8. Foxp2 : forkhead box P2. Foxq1 : forkhead box Q1. Gdf5 : growth differentiation factor 5. Gfra4 : glial cell line derived neurotrophic factor family receptor alpha 4. Igfbp2 : insulin-like growth factor binding protein 2. Igfbp7 : insulin-like growth factor binding protein 7. Klf14 : Kruppel-like transcription factor 14. Neurod6 : neurogenic differentiation 6. Notch4 : notch 4. Nupr1 : nuclear protein transcription regulator 1. Reg3b : regenerating islet-derived 3 beta. Rgmb : repulsive guidance molecule family member B. Sp8 : trans - acting transcription factor 8. Spdef : SAM pointed domain containing ets transcription factor. Tcfl5 : transcription factor-like 5 (basic helix-loop-helix). Tfap2b : transcription factor AP-2 beta. Wif1 : Wnt inhibitory factor 1. Barhl1 : BarH like homeobox 1. Barhl2 : BarH like homeobox 2. Dlx3 : distal-less homeobox 3. Hhex : hematopoietically expressed homeobox. Hdx : highly divergent homeobox. Hoxb6 : homeobox B6. Hoxb7 : homeobox B7. Hoxc5 : homeobox C5. Hoxc11 : homeobox C11. Hoxd11 : homeobox D11. Hopx : HOP homeobox. Lhx5 : LIM homeobox protein 5. Otp : orthopedia homeobox. Vsx2 : visual system homeobox 2. Aven : apoptosis, caspase activation inhibitor. Casp6 : caspase 6. Pawr : PRKC, apoptosis, WT1, regulator. Raw data are available under the following link: https://doi.org/10.57745/WE0VK8 .

Article Snippet: KIR2.2 receptor , Anti-K ir 2.2 (KCNJ12) , Rabbit , 1:500 , Alomone (APC-042).

Techniques: RNA Sequencing, Selection, Expressing, Derivative Assay, Binding Assay, Activation Assay

Journal: PLOS Biology

Article Title: Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice

doi: 10.1371/journal.pbio.3003153

Figure Lengend Snippet: A. Representative traces of the current recorded during the application of a long voltage ramp from −40 mV to −150 mV in MNs held at −40 mV in control condition (black trace) and in the presence of 500 μm Ba 2+ (blue trace) (A1). The mean cord conductance of the current was measured at membrane potentials equally distant from the reversal potential (Erev), Erev+40 and Erev-40. Violin plots of the chord conductances measured in untrained (black, n = 40) and trained (purple, n = 36) MNs (A2). Violin plots of the chord conductance of the current suppressed by Ba 2+ in untrained (black, n = 15) and trained (purple, n = 17) MNs (A3). * Significantly different; *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA analysis, followed by uncorrected Fisher’s LSD post-tests. B. Representative Kir2.2 (green) and GAPDH (red) bands from ventral lumbar spinal cords of untrained and trained P3 mice (top panel), with the quantitative results of western blotting analysis (bottom panel). C. Sample membrane current traces obtained in response to negative voltage steps in a MN held at −60 mV in voltage clamp conditions. The I H current was computed by subtracting the instantaneous current (■) from the steady state (☐) (E1). Mean instantaneous I-V curves derived from the current responses generated by a series of voltage steps in untrained (black dots, n = 14) and trained (purple dots, n = 17) MNs (E2). Underlying data can be found in the Sheet.

Article Snippet: KIR2.2 receptor , Anti-K ir 2.2 (KCNJ12) , Rabbit , 1:500 , Alomone (APC-042).

Techniques: Control, Membrane, Western Blot, Derivative Assay, Generated