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Validation of <t>FLNB-regulated</t> genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of <t>ITGA10,</t> <t>CEBP/β,</t> and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
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Validation of <t>FLNB-regulated</t> genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of <t>ITGA10,</t> <t>CEBP/β,</t> and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
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Validation of <t>FLNB-regulated</t> genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of <t>ITGA10,</t> <t>CEBP/β,</t> and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
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Validation of <t>FLNB-regulated</t> genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of <t>ITGA10,</t> <t>CEBP/β,</t> and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
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Validation of <t>FLNB-regulated</t> genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of <t>ITGA10,</t> <t>CEBP/β,</t> and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
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Image Search Results


Validation of FLNB-regulated genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of ITGA10, CEBP/β, and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.

Journal: Heliyon

Article Title: Filamin B knockdown impairs differentiation and function in mouse pre-osteoblasts via aberrant transcription and alternative splicing

doi: 10.1016/j.heliyon.2024.e39334

Figure Lengend Snippet: Validation of FLNB-regulated genes (DEGs). A) Expression levels of osteogenesis related up-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). B) Expression levels of osteogenesis-associated down-regulated DEG after knockdown of FLNB (FPKM, up) and RT-qPCR measurements (down). C) Protein expression levels of ITGA10, CEBP/β, and GREM1 genes. Compared with sh-NC group, ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.

Article Snippet: Then, the membrane was incubated with the appropriate antibody concentration at 4 °C overnight (FLNB, 1:1000, Proteintech; CEBP/β,1:1000, Affitiny; GREM1,1:1000, Affitiny; ITGA10,1:1000, Affitiny; GAPDH,1:5000, Proteintech).

Techniques: Biomarker Discovery, Expressing, Knockdown, Quantitative RT-PCR