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FIGURE 3 | Proliferation and DNA repair activity. Immunolabelling for PCNA and PARP1 (in green) and <t>BAP1</t> (in red): In the control condition and differently treated GSCs, that is, after 48 h-CT Cisplatin 40 μM or Pt(IV)Ac-POA 10 μM. DNA was stained with Hoechst 33,258 (blue fluores- cence). Magnification 25×, bar of 40 μm. The histograms show the mean fluorescence intensity value of the immunolabelling ± SEM for (A) PCNA, (B) PARP1, and (C) BAP1. Statistical significance calculated as follows: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.1.
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FIGURE 3 | Proliferation and DNA repair activity. Immunolabelling for PCNA and PARP1 (in green) and <t>BAP1</t> (in red): In the control condition and differently treated GSCs, that is, after 48 h-CT Cisplatin 40 μM or Pt(IV)Ac-POA 10 μM. DNA was stained with Hoechst 33,258 (blue fluores- cence). Magnification 25×, bar of 40 μm. The histograms show the mean fluorescence intensity value of the immunolabelling ± SEM for (A) PCNA, (B) PARP1, and (C) BAP1. Statistical significance calculated as follows: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.1.
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FIGURE 3 | Proliferation and DNA repair activity. Immunolabelling for PCNA and PARP1 (in green) and <t>BAP1</t> (in red): In the control condition and differently treated GSCs, that is, after 48 h-CT Cisplatin 40 μM or Pt(IV)Ac-POA 10 μM. DNA was stained with Hoechst 33,258 (blue fluores- cence). Magnification 25×, bar of 40 μm. The histograms show the mean fluorescence intensity value of the immunolabelling ± SEM for (A) PCNA, (B) PARP1, and (C) BAP1. Statistical significance calculated as follows: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.1.
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FIGURE 3 | Proliferation and DNA repair activity. Immunolabelling for PCNA and PARP1 (in green) and <t>BAP1</t> (in red): In the control condition and differently treated GSCs, that is, after 48 h-CT Cisplatin 40 μM or Pt(IV)Ac-POA 10 μM. DNA was stained with Hoechst 33,258 (blue fluores- cence). Magnification 25×, bar of 40 μm. The histograms show the mean fluorescence intensity value of the immunolabelling ± SEM for (A) PCNA, (B) PARP1, and (C) BAP1. Statistical significance calculated as follows: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.1.
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FIGURE 3 | Proliferation and DNA repair activity. Immunolabelling for PCNA and PARP1 (in green) and BAP1 (in red): In the control condition and differently treated GSCs, that is, after 48 h-CT Cisplatin 40 μM or Pt(IV)Ac-POA 10 μM. DNA was stained with Hoechst 33,258 (blue fluores- cence). Magnification 25×, bar of 40 μm. The histograms show the mean fluorescence intensity value of the immunolabelling ± SEM for (A) PCNA, (B) PARP1, and (C) BAP1. Statistical significance calculated as follows: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.1.

Journal: Cell proliferation

Article Title: DNA Damage Repair in Glioblastoma: A Novel Approach to Combat Drug Resistance.

doi: 10.1111/cpr.13815

Figure Lengend Snippet: FIGURE 3 | Proliferation and DNA repair activity. Immunolabelling for PCNA and PARP1 (in green) and BAP1 (in red): In the control condition and differently treated GSCs, that is, after 48 h-CT Cisplatin 40 μM or Pt(IV)Ac-POA 10 μM. DNA was stained with Hoechst 33,258 (blue fluores- cence). Magnification 25×, bar of 40 μm. The histograms show the mean fluorescence intensity value of the immunolabelling ± SEM for (A) PCNA, (B) PARP1, and (C) BAP1. Statistical significance calculated as follows: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.1.

Article Snippet: Antigen Primary antibody Dilution PCNA Mouse monoclonal [PC10] antiPCNA (Abcam, Cambridge, UK) 1:200 PARP- 1 Rabbit polyclonal antiPARP- 1 (Cell Signalling Technology, Danvers, USA) 1:200 BAP- 1 Mouse monoclonal anti- BAP1 (Santa Cruz Biotechnology, Dallas, USA) 1:200 HIF- 1α Rabbit monoclonal anti- HIF- 1α (D1S7W) (Cell Signalling Technology, Danvers, USA) 1:200 AIF Rabbit polyclonal anti- AIF (Cell Signalling Technology, Danvers, USA) 1:200 COX- 2 Mouse polyclonal antiCOX2 (M- 19) (Santa Cruz Biotechnology, Dallas, USA) 1:200 SOD- 2 Rabbit monoclonal antiSOD2 (Cell Signalling Technology, Danvers, USA) 1:200 GPx- 4 Rabbit polyclonal anti- GPx4 (Abcam, Cambridge, UK) 1:200 13652184, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/cpr.13815 by IN A SP - N E PA L , W iley O nline L ibrary on [02/02/2025].

Techniques: Activity Assay, Control, Staining, Fluorescence