Journal: PLOS One

Article Title: Cellular repressor of E1A-stimulated genes 1 enhances skeletal muscle performance through the stimulation of muscle differentiation and Akt-mTOR signaling pathway activation

doi: 10.1371/journal.pone.0328485

Figure Lengend Snippet: (A) Western blotting for CREG1 was performed using the serum (1 µL) of wild type (WT) and Tg mice. (B-C) The expression of CREG1 in the soleus muscles of WT and Tg mice was examined using RT-PCR and western blot analysis. Relative levels of Creg1 mRNA (B) and CREG1 protein (C) in the soleus muscles. (D) Relative levels of insulin-like growth factor 2 receptor (IGF2R), phosphorylated Akt Ser 473 (pAkt), total Akt (Akt), phosphorylated mechanistic target of rapamycin (pmTOR) Ser 2448 and total mTOR (mTOR) in the soleus muscles. Data were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (E) Western blotting for IGF2R, pAkt, Akt, pmTOR, mTOR and GAPDH in the soleus muscles. Representative images are shown. Data are presented as mean ± SEM; n = 4–6 per group. A student's t -test was performed; * P < 0.05, versus WT.

Article Snippet: The membranes were blocked for 1 h at room temperature in nonfat dry milk and then incubated overnight at 4 °C with the following antibodies: anti-CREG1 (ab233282; Abcam, Cambridge, UK), anti-Akt Ser 473 [9271; Cell Signaling Technology (CST), Danvers, MA, USA], anti-Akt (9272; CST), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2118; CST), anti- insulin-like growth factor 2 receptor (IGF2R) (14364; CST), anti- mTOR Ser 2448 (2971; CST), anti- mTOR (2972; CST), and anti-α/β-tubulin (2148; CST).

Techniques: Western Blot, Expressing, Muscles, Reverse Transcription Polymerase Chain Reaction

Journal: Traffic (Copenhagen, Denmark)

Article Title: Acute GARP Depletion Disrupts Vesicle Transport, Leading to Severe Defects in Sorting, Secretion and O ‐Glycosylation

doi: 10.1111/tra.70003

Figure Lengend Snippet: List of primary and secondary antibodies.

Article Snippet: CD‐M6PR/MPR , Santa Cruz, #sc365196 , Mouse , 1:1000 , —.

Techniques: Transduction