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Heat stress-induced ferroptosis in the liver. ( A ) Serum Fe 2+ and total iron levels. ( B ) Liver Fe 2+ and total iron levels. ( C ) Protein results graph. ( D – J ) Analysis of protein results. ( K – M ) Immunohistochemical results and analysis for CD71 and <t>PTGS2.</t> All data were expressed as mean ± SEM; “*” indicates statistically significant difference with the CON group (* p < 0.05, ** p < 0.01, *** p < 0.01).
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Heat stress-induced ferroptosis in the liver. ( A ) Serum Fe 2+ and total iron levels. ( B ) Liver Fe 2+ and total iron levels. ( C ) Protein results graph. ( D – J ) Analysis of protein results. ( K – M ) Immunohistochemical results and analysis for CD71 and <t>PTGS2.</t> All data were expressed as mean ± SEM; “*” indicates statistically significant difference with the CON group (* p < 0.05, ** p < 0.01, *** p < 0.01).
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Heat stress-induced ferroptosis in the liver. ( A ) Serum Fe 2+ and total iron levels. ( B ) Liver Fe 2+ and total iron levels. ( C ) Protein results graph. ( D – J ) Analysis of protein results. ( K – M ) Immunohistochemical results and analysis for CD71 and <t>PTGS2.</t> All data were expressed as mean ± SEM; “*” indicates statistically significant difference with the CON group (* p < 0.05, ** p < 0.01, *** p < 0.01).
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Heat stress-induced ferroptosis in the liver. ( A ) Serum Fe 2+ and total iron levels. ( B ) Liver Fe 2+ and total iron levels. ( C ) Protein results graph. ( D – J ) Analysis of protein results. ( K – M ) Immunohistochemical results and analysis for CD71 and PTGS2. All data were expressed as mean ± SEM; “*” indicates statistically significant difference with the CON group (* p < 0.05, ** p < 0.01, *** p < 0.01).

Journal: Veterinary Sciences

Article Title: Heat Stress in the Liver of Chicken: Insights from Keap1-Nrf2 Pathway Mediated Ferroptosis and Cuproptosis via the HO-1/FDX1/Gpx4 Axis

doi: 10.3390/vetsci13060512

Figure Lengend Snippet: Heat stress-induced ferroptosis in the liver. ( A ) Serum Fe 2+ and total iron levels. ( B ) Liver Fe 2+ and total iron levels. ( C ) Protein results graph. ( D – J ) Analysis of protein results. ( K – M ) Immunohistochemical results and analysis for CD71 and PTGS2. All data were expressed as mean ± SEM; “*” indicates statistically significant difference with the CON group (* p < 0.05, ** p < 0.01, *** p < 0.01).

Article Snippet: Subsequent to this, the incubation process was continued for a period of 16 h, during which the following antibodies were utilized: CD71 (1:1000, Servicebio, Wuhan, China), PTGS2 (1:200, ABclonal, Wuhan, China) and ATP7B (1:200, Zenbio, Chengdu, China).

Techniques: Immunohistochemical staining