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VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Aml12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Mouse Hepatocyte Cell Line Aml12, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aml12+cells/AML12/pm42778596-436-11-20
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mouse hepatocyte cell line aml12 - by Bioz Stars, 2026-10
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aml12 cell lines  (ATCC)
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ATCC aml12 cell lines
VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Aml12 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cometassay single cell gel electrophoresis assay  (Bio-Techne corporation)
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VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Cometassay Single Cell Gel Electrophoresis Assay, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse liver cell line aml12  (Procell Inc)
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Procell Inc mouse liver cell line aml12
VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
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thle 2 cell culture aml12 cells  (ATCC)
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VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Thle 2 Cell Culture Aml12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine hepatic aml12 cells  (Procell Inc)
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Procell Inc murine hepatic aml12 cells
VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
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murine hepatic aml12 cells - by Bioz Stars, 2026-10
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murine hepatocyte cell line aml12  (ATCC)
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ATCC murine hepatocyte cell line aml12
VB12 can alleviate H/R-induced damage in <t>AML12</t> cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Murine Hepatocyte Cell Line Aml12, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aml12+cells/AML12/pm42249087-64-1-6
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murine hepatocyte cell line aml12 - by Bioz Stars, 2026-10
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VB12 can alleviate H/R-induced damage in AML12 cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Journal: Journal of Inflammation Research

Article Title: Vitamin B12 Alleviates Hepatic Ischemia–Reperfusion Injury with Restoration of Propanoate Metabolism and Mitochondrial Function

doi: 10.2147/JIR.S616890

Figure Lengend Snippet: VB12 can alleviate H/R-induced damage in AML12 cells. ( A ) Schematic diagram of the experimental procedure. ( B ) Alamar Blue assay to detect cell viability of hepatocytes after H/R treatment with different concentrations of vitamin B12 (0.3–300μM). ( C ) Flow cytometry assay to detect the proportion of Annexin V + apoptotic cells and Ki67 + proliferating cells, and the MFI of IL-6 and IL-1β. ( D ) qRT-PCR assay to detect the mRNA expression levels of Il6, Il1b, Tnf, Ccl2 and Cxcl2 . Data are expressed as mean ± SEM (n = 4 per group). Significance indicators, *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Article Snippet: AML12 cells were obtained from the American Type Culture Collection (ATCC; CRL-2254) and cultured in DMEM/F12 medium containing 10% fetal bovine serum (FBS) and supplemented with ITS and dexamethasone.

Techniques: Alamar Blue Assay, Flow Cytometry, Quantitative RT-PCR, Expressing