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The effect of increased weight loading on body weight is partly dependent on TrkA mediated signaling. ( a – d ) The effect of increased loading on body weight in mice with either intact peripheral nerve signaling ( TrkA WT ) or mice lacking functional TrkA signaling ( TrkA F592A ). ( a ) Male TrkA WT mice (control n = 7, load n = 7), ( b ) male TrkA F592A mice (control n = 7, load n = 6), ( c ) female TrkA WT mice (control n = 8, load n = 10), and ( d ) female TrkA F592A mice (control n = 7, load n = 5). ( e , f ) The effect of increased weight loading on retroperitoneal white adipose tissue (WAT) weight in male ( e ) and female ( f ) mice. Data are shown as mean ± SEM. Statistical analyses were performed using repeated measures ANOVA (for weight curves) or Student’s t-test (for retroperitoneal WAT). p < 0.05 was considered significant., n.s = non-significant.

Journal: Scientific Reports

Article Title: The effect of increased weight loading on body weight is partly dependent on Piezo1 in osteoblast-lineage cells and TrkA signaling

doi: 10.1038/s41598-026-40431-8

Figure Lengend Snippet: The effect of increased weight loading on body weight is partly dependent on TrkA mediated signaling. ( a – d ) The effect of increased loading on body weight in mice with either intact peripheral nerve signaling ( TrkA WT ) or mice lacking functional TrkA signaling ( TrkA F592A ). ( a ) Male TrkA WT mice (control n = 7, load n = 7), ( b ) male TrkA F592A mice (control n = 7, load n = 6), ( c ) female TrkA WT mice (control n = 8, load n = 10), and ( d ) female TrkA F592A mice (control n = 7, load n = 5). ( e , f ) The effect of increased weight loading on retroperitoneal white adipose tissue (WAT) weight in male ( e ) and female ( f ) mice. Data are shown as mean ± SEM. Statistical analyses were performed using repeated measures ANOVA (for weight curves) or Student’s t-test (for retroperitoneal WAT). p < 0.05 was considered significant., n.s = non-significant.

Article Snippet: Immediately after the experiment, the mice received an intraperitoneal injection of the TrkA agonist gambogic amide (Alomone Lab #G-235), 4 mg/kg to induce peripheral sensitization .

Techniques: Functional Assay, Control