Journal: International Dental Journal

Article Title: MZB1 Drives Periodontitis via ER Stress-Mediated Inflammation and Osteogenic Differentiation

doi: 10.1016/j.identj.2026.109452

Figure Lengend Snippet: Knockdown of MZB1 restores osteogenic differentiation capacity of PDLSCs under inflammatory conditions (A) Microscopic images of alizarin red S staining showing calcium nodule formation after osteogenic induction of PDLSCs under different conditions. (B) Quantification of alizarin red S staining by absorbance at 560 nm (N = 3). (C) Quantitative analysis of alkaline phosphatase (ALP) activity following osteogenic induction of PDLSCs (N = 3). (D) Western blot images of osteogenic differentiation-related proteins (RUNX2, BMP2, α-SMA, and Collagen I) in PDLSCs. (E) Quantification of Western blot bands normalised to β-Actin, representing relative expression levels of osteogenic markers (N = 3).

Article Snippet: After determining protein concentrations, ALP activity was measured using an ALP activity assay kit (Nanjing Jiancheng Bioengineering Institute, China).

Techniques: Knockdown, Staining, Activity Assay, Western Blot, Expressing

Journal: Fundamental Research

Article Title: Melatonin ameliorates inflammation-induced mitochondrial dysfunction and cementoblastic differentiation in cells by regulating the METTL3/LINC01444/HSPD1 axis

doi: 10.1016/j.fmre.2024.05.012

Figure Lengend Snippet: Melatonin ameliorates inflammation-induced damage to the cementoblastic differentiation of PDLSCs . PDLSCs were cultured in cementoblastic medium (Nor), cementoblastic medium supplemented with inflammatory cytokines (Infla), or cementoblastic medium supplemented with inflammatory cytokines (plus 5 µM, 25 µM or 100 µM melatonin; Infla + Mel). (a) Diagram depicting the construction of the inflammatory environment and the melatonin treatment schedule for the in vitro model; (b) Relative mRNA expression levels of the cementoblastic markers ( BSP, CAP and CEMP-1 ) were measured by qRT‒PCR; (c) Relative protein expression levels of the cementoblastic markers (BSP, CAP and CEMP-1) were detected by Western blotting; (d) Representative images of ALP staining of PDLSCs (scale bar: 500 µm); (e) Quantitative analysis of ALP activity. The results are presented as the means ± SDs ( n = 3–12); P values (* P < 0.05, ** P < 0.01 and *** P < 0.001) were calculated by one-way ANOVA.

Article Snippet: ALP activity in the collected supernatants was tested using an ALP activity detection kit (Jiancheng Bioengineering, Nanjing, China).

Techniques: Cell Culture, In Vitro, Expressing, Western Blot, Staining, Activity Assay