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Crebanine (Cre) prevents ovariectomy (OVX)-induced bone loss. (A) Schematic illustration of the experimental design evaluating Cre's therapeutic efficacy in vivo . (B) Representative micro-computed tomography (CT) images of distal femoral structures in each treatment group. (C–G) Quantitative measurements of bone microarchitecture parameters including bone parameters including bone volume/tissue volume (BV/TV) (C), trabecular number (Tb.N) (D), trabecular separation (Tb.Sp) (E), trabecular thickness (Tb.Th) (F), and bone mineral density (BMD) (G) of five groups ( n = 6) by CTAn software. (H) Representative hematoxylin and eosin (H&E) staining images of distal femurs sections in each group. (I) Quantitative analyses of BV/TV ( n = 6). Data represent means ± standard error of the mean (SEM). Statistical significance was determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. ∗ P < 0.05 and ∗∗ P < 0.01. Aln: <t>alendronate;</t> TRAP: tartrate-resistant acid phosphatase; ELISA: enzyme-linked immunosorbent assay; β-CTx: type I collagen cross-linked C-terminal telopeptide; IHC: immunohistochemistry; Sirt1: sirtuin 1.
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Crebanine (Cre) prevents ovariectomy (OVX)-induced bone loss. (A) Schematic illustration of the experimental design evaluating Cre's therapeutic efficacy in vivo . (B) Representative micro-computed tomography (CT) images of distal femoral structures in each treatment group. (C–G) Quantitative measurements of bone microarchitecture parameters including bone parameters including bone volume/tissue volume (BV/TV) (C), trabecular number (Tb.N) (D), trabecular separation (Tb.Sp) (E), trabecular thickness (Tb.Th) (F), and bone mineral density (BMD) (G) of five groups ( n = 6) by CTAn software. (H) Representative hematoxylin and eosin (H&E) staining images of distal femurs sections in each group. (I) Quantitative analyses of BV/TV ( n = 6). Data represent means ± standard error of the mean (SEM). Statistical significance was determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. ∗ P < 0.05 and ∗∗ P < 0.01. Aln: alendronate; TRAP: tartrate-resistant acid phosphatase; ELISA: enzyme-linked immunosorbent assay; β-CTx: type I collagen cross-linked C-terminal telopeptide; IHC: immunohistochemistry; Sirt1: sirtuin 1.

Journal: Journal of Pharmaceutical Analysis

Article Title: Crebanine protects against ovariectomy-induced bone loss by targeting Sirt1 to interfere with NF-κB acetylation and ROS activity

doi: 10.1016/j.jpha.2025.101426

Figure Lengend Snippet: Crebanine (Cre) prevents ovariectomy (OVX)-induced bone loss. (A) Schematic illustration of the experimental design evaluating Cre's therapeutic efficacy in vivo . (B) Representative micro-computed tomography (CT) images of distal femoral structures in each treatment group. (C–G) Quantitative measurements of bone microarchitecture parameters including bone parameters including bone volume/tissue volume (BV/TV) (C), trabecular number (Tb.N) (D), trabecular separation (Tb.Sp) (E), trabecular thickness (Tb.Th) (F), and bone mineral density (BMD) (G) of five groups ( n = 6) by CTAn software. (H) Representative hematoxylin and eosin (H&E) staining images of distal femurs sections in each group. (I) Quantitative analyses of BV/TV ( n = 6). Data represent means ± standard error of the mean (SEM). Statistical significance was determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. ∗ P < 0.05 and ∗∗ P < 0.01. Aln: alendronate; TRAP: tartrate-resistant acid phosphatase; ELISA: enzyme-linked immunosorbent assay; β-CTx: type I collagen cross-linked C-terminal telopeptide; IHC: immunohistochemistry; Sirt1: sirtuin 1.

Article Snippet: Cre (C 20 H 21 NO 4 , purity ≥ 99.54%, HY-N2255) and alendronate (Aln) (C 4 H 12 NNaO 7 P 2, purity ≥ 98.0%, HY-108685) were commercially sourced from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Drug discovery, In Vivo, Micro-CT, Software, Staining, Comparison, Enzyme-linked Immunosorbent Assay, Immunohistochemistry