Journal: Applied and Environmental Microbiology

Article Title: Mycolic Acid-Containing Bacteria Induce Natural-Product Biosynthesis in Streptomyces Species ▿ †

doi: 10.1128/AEM.01337-10

Figure Lengend Snippet: Production of a novel antibiotic, alchivemycin A, in a combined culture of Streptomyces endus S-522 and T. pulmonis or C. glutamicum. Shown is the production of alchivemycin A in a combined culture of S. endus S-522 and T. pulmonis or C. glutamicum. (A to D) HPLC profiles of the secondary metabolites produced by S-522 cultured with or without T. pulmonis. Shown are the culture broths of the combined culture with T. pulmonis (A), combined culture with C. glutamicum (B), S-522 pure culture (C), and T. pulmonis pure culture (D). The arrow shows the peak for alchivemycin A. Elution was performed with a linear gradient, as indicated on the scale at the right. (E) Chemical structure of alchivemycin A.

Article Snippet: This antibiotic contains a heterocyclic chromophore that has not been described previously and shows specific antibiotic activity against Micrococcus luteus at a concentration of 0.06 μg/ml (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Organism Relevant characteristic Alchivemycin A MIC (μg/ml) Micrococcus luteus Gram positive 0.06 Bacillus subtilis Gram positive 40 Staphylococcus aureus Gram positive >50 Escherichia coli Gram negative >50 Saccharomyces cerevisiae Yeast cell >50 Candida albicans Yeast cell >50 Open in a separate window a The MICs were determined by the 2-fold sequential dilution method in heart infusion broth (Difco).

Techniques: Produced, Cell Culture

Journal: Applied and Environmental Microbiology

Article Title: Mycolic Acid-Containing Bacteria Induce Natural-Product Biosynthesis in Streptomyces Species ▿ †

doi: 10.1128/AEM.01337-10

Figure Lengend Snippet: Antibacterial activities of alchivemycin A a

Article Snippet: This antibiotic contains a heterocyclic chromophore that has not been described previously and shows specific antibiotic activity against Micrococcus luteus at a concentration of 0.06 μg/ml (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Organism Relevant characteristic Alchivemycin A MIC (μg/ml) Micrococcus luteus Gram positive 0.06 Bacillus subtilis Gram positive 40 Staphylococcus aureus Gram positive >50 Escherichia coli Gram negative >50 Saccharomyces cerevisiae Yeast cell >50 Candida albicans Yeast cell >50 Open in a separate window a The MICs were determined by the 2-fold sequential dilution method in heart infusion broth (Difco).

Techniques: