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Reduced pancreatic ORM2 expression in AP. (A) Scatterplots showing log2 transformation of the normalized blood <t>ORM1</t> in patients with MAP (n=57) and healthy controls (n=32). (B) Scatterplots showing log2 transformation of the normalized blood ORM2 in patients with MAP (n=57) and healthy controls (n=32). (C) The serum ORM in control and AP mice (n=6 per group). (D) Immunoblotting of ORM1 and ORM2 in serum from control and AP mice (n=6 per group). (E) Immunoblotting and quantitative analysis of ORM1 and ORM2 in pancreas from control and AP mice (n=6 per group). (F) Immunoblotting and quantitative analysis of ORM1 and ORM2 in liver from control and AP mice (n=6 per group). Data are presented as mean ± SEM. The groups were compared using unpaired two-tailed student’s t -test (*, P<0.05; ***, P<0.001; ****, P<0.0001). AP, acute pancreatitis; MAP, mild acute pancreatitis; ORM, orosomucoid; SEM, standard error of the mean.
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Reduced pancreatic ORM2 expression in AP. (A) Scatterplots showing log2 transformation of the normalized blood <t>ORM1</t> in patients with MAP (n=57) and healthy controls (n=32). (B) Scatterplots showing log2 transformation of the normalized blood ORM2 in patients with MAP (n=57) and healthy controls (n=32). (C) The serum ORM in control and AP mice (n=6 per group). (D) Immunoblotting of ORM1 and ORM2 in serum from control and AP mice (n=6 per group). (E) Immunoblotting and quantitative analysis of ORM1 and ORM2 in pancreas from control and AP mice (n=6 per group). (F) Immunoblotting and quantitative analysis of ORM1 and ORM2 in liver from control and AP mice (n=6 per group). Data are presented as mean ± SEM. The groups were compared using unpaired two-tailed student’s t -test (*, P<0.05; ***, P<0.001; ****, P<0.0001). AP, acute pancreatitis; MAP, mild acute pancreatitis; ORM, orosomucoid; SEM, standard error of the mean.
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Reduced pancreatic ORM2 expression in AP. (A) Scatterplots showing log2 transformation of the normalized blood <t>ORM1</t> in patients with MAP (n=57) and healthy controls (n=32). (B) Scatterplots showing log2 transformation of the normalized blood ORM2 in patients with MAP (n=57) and healthy controls (n=32). (C) The serum ORM in control and AP mice (n=6 per group). (D) Immunoblotting of ORM1 and ORM2 in serum from control and AP mice (n=6 per group). (E) Immunoblotting and quantitative analysis of ORM1 and ORM2 in pancreas from control and AP mice (n=6 per group). (F) Immunoblotting and quantitative analysis of ORM1 and ORM2 in liver from control and AP mice (n=6 per group). Data are presented as mean ± SEM. The groups were compared using unpaired two-tailed student’s t -test (*, P<0.05; ***, P<0.001; ****, P<0.0001). AP, acute pancreatitis; MAP, mild acute pancreatitis; ORM, orosomucoid; SEM, standard error of the mean.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Reduced pancreatic ORM2 expression in AP. (A) Scatterplots showing log2 transformation of the normalized blood ORM1 in patients with MAP (n=57) and healthy controls (n=32). (B) Scatterplots showing log2 transformation of the normalized blood ORM2 in patients with MAP (n=57) and healthy controls (n=32). (C) The serum ORM in control and AP mice (n=6 per group). (D) Immunoblotting of ORM1 and ORM2 in serum from control and AP mice (n=6 per group). (E) Immunoblotting and quantitative analysis of ORM1 and ORM2 in pancreas from control and AP mice (n=6 per group). (F) Immunoblotting and quantitative analysis of ORM1 and ORM2 in liver from control and AP mice (n=6 per group). Data are presented as mean ± SEM. The groups were compared using unpaired two-tailed student’s t -test (*, P<0.05; ***, P<0.001; ****, P<0.0001). AP, acute pancreatitis; MAP, mild acute pancreatitis; ORM, orosomucoid; SEM, standard error of the mean.

Journal: Translational Gastroenterology and Hepatology

Article Title: ORM2 protects against acute pancreatitis by inhibiting premature activation of pancreatic enzymes

doi: 10.21037/tgh-25-106

Figure Lengend Snippet: Reduced pancreatic ORM2 expression in AP. (A) Scatterplots showing log2 transformation of the normalized blood ORM1 in patients with MAP (n=57) and healthy controls (n=32). (B) Scatterplots showing log2 transformation of the normalized blood ORM2 in patients with MAP (n=57) and healthy controls (n=32). (C) The serum ORM in control and AP mice (n=6 per group). (D) Immunoblotting of ORM1 and ORM2 in serum from control and AP mice (n=6 per group). (E) Immunoblotting and quantitative analysis of ORM1 and ORM2 in pancreas from control and AP mice (n=6 per group). (F) Immunoblotting and quantitative analysis of ORM1 and ORM2 in liver from control and AP mice (n=6 per group). Data are presented as mean ± SEM. The groups were compared using unpaired two-tailed student’s t -test (*, P<0.05; ***, P<0.001; ****, P<0.0001). AP, acute pancreatitis; MAP, mild acute pancreatitis; ORM, orosomucoid; SEM, standard error of the mean.

Article Snippet: The antibodies used in this study are listed as follows: ORM1 (No. 16439-1-AP proteintech, Wuhan, China), ORM2 (No. P19652 , zenbio, Chengdu, China), GAPDH (No. A19056, ABclonal, Wuhan, China), HRP-conjugated Goat anti-Rabbit IgG (H+L) (No. AS014, ABclonal, Wuhan, China).

Techniques: Expressing, Transformation Assay, Control, Western Blot, Two Tailed Test

Some representative chromatograms from the HPLC study on a chiral AGP column at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.

Journal: ACS Omega

Article Title: Characterization of the Interaction between Human α 1 ‑Acid Glycoprotein and Apremilast Enantiomers: HPLC, Isothermal Titration Calorimetry, Ultracentrifugation, and Docking Studies

doi: 10.1021/acsomega.5c07756

Figure Lengend Snippet: Some representative chromatograms from the HPLC study on a chiral AGP column at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.

Article Snippet: The chiral AGP column (ChromTech Chiral-AGP) with dimensions of 150 mm × 4.0 mm was sourced from ChromTech Ltd. (Congleton, UK).

Techniques: