Journal: Frontiers in Immunology

Article Title: Prognostic analysis and identification of M7G immune-related genes in lung squamous cell carcinoma

doi: 10.3389/fimmu.2025.1515838

Figure Lengend Snippet: Validation of clinical samples. (A) Immunohistochemistry was employed to detect the expression levels of FGA, CSF3R, ORM1, and NTS. (B) Survival analysis of m7G immune-related gene expression, risk stratification, and their correlation with the prognosis of lung squamous cell carcinoma patients. The small letters indicates the scale bar that represents 150 μm.

Article Snippet: The following antibodies were used in this study: CSF3R (Cusabio, China); NTS (821026, Proteintech, China); ORM1 (16439-1-AP, ZEN-BIOSCIENCE, China); FGA (20645-1-AP, Proteintech, China).

Techniques: Biomarker Discovery, Immunohistochemistry, Expressing, Gene Expression

Journal: Frontiers in Immunology

Article Title: Prognostic analysis and identification of M7G immune-related genes in lung squamous cell carcinoma

doi: 10.3389/fimmu.2025.1515838

Figure Lengend Snippet: Correlation between the clinicopathological characteristics and expression of ORM1 in LUSC.

Article Snippet: The following antibodies were used in this study: CSF3R (Cusabio, China); NTS (821026, Proteintech, China); ORM1 (16439-1-AP, ZEN-BIOSCIENCE, China); FGA (20645-1-AP, Proteintech, China).

Techniques: Expressing

Journal: The FASEB Journal

Article Title: Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas’ disease progression

doi: 10.1096/fj.202200427rr

Figure Lengend Snippet: FIGURE 3 Phosphorylation analysis revealed distinct protein signatures in different groups. (A) Hierarchical clustering analysis of 152 LFQ-phosphorylation intensities (log2) quantified in three repeats of the four groups' proteomes and showed at least p < 0.05 in their abundance between at least two groups. (B) GO enrichment analysis for the category of upregulated and downregulated phosphorylated proteins between sham and OVX groups. (C) GO enrichment analysis for the category of upregulated and downregulated phosphorylated proteins between sham and MENO groups. (D) MAPK signaling pathway: signaling pathways associated with differentiation, proliferation, and inflammation in the menopausal group. (E) Ether lipid metabolism: signaling pathways associated with lipid metabolism in the menopausal group. (F) The mRNA levels of RSK2, JNK, and LPCAT4 were detected in the LN229 cells with estrogen stimulation. Statistical analysis was performed by unpaired T tests, **p < 0.01 compared with the control group. (G) LPCAT4 protein levels were detected in the LN229 cells with estrogen stimulation compared with no-estrogen stimulation. Statistical analysis was performed by unpaired T tests, **p < 0.01 compared with the control group.

Article Snippet: Primary antibodies, rabbit anti JNK (pT183/pY185) (4668, cell signaling), and rabbit anti LPCAT4 (17905- 1- AP, Proteintech) were used to analyze the protein expression levels.

Techniques: Phospho-proteomics, Protein-Protein interactions, Control

Journal: The FASEB Journal

Article Title: Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas’ disease progression

doi: 10.1096/fj.202200427rr

Figure Lengend Snippet: FIGURE 5 Identification of upregulated and downregulated proteins in human gliomas. (A) The differentially expressed proteins (top 20) from the postmenopausal brain proteomics database of mice. (B) Expression levels of upregulated and downregulated proteins in human gliomas from TCGA datasets. (C) Overall survival curves for the expression of ACTA2, CAMK2D, FNBP1L, ARL1, HEBP1, CAST, CLIC1, LPCAT4, MAST3, and DOCK9 in Gliomas (Kaplan–Meier Plotter). (D) Correlation analysis of ACTA2, CAMK2D, FNBP1L, ARL1, HEBP1, CAST, CLIC1, LPCAT4, MAST3, and DOCK9 gene expression TMB/MSI. The horizontal axis in the figure represents the expression distribution of the gene, and the ordinate is the expression distribution of the TMB/MSI score. The density curve on the right represents the distribution trend of the TMB/MSI score; the upper-density curve represents the distribution trend of the gene on the top side. The value represents the correlation p-value, correlation coefficient, and correlation calculation method. (E and F) Expression levels of upregulated and downregulated proteins in human gliomas from TCGA datasets by multi-factor analysis including ages and grades.

Article Snippet: Primary antibodies, rabbit anti JNK (pT183/pY185) (4668, cell signaling), and rabbit anti LPCAT4 (17905- 1- AP, Proteintech) were used to analyze the protein expression levels.

Techniques: Expressing, Gene Expression

Journal: The FASEB Journal

Article Title: Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas’ disease progression

doi: 10.1096/fj.202200427rr

Figure Lengend Snippet: FIGURE 7 Identification of upregulated and downregulated genes in human postmenopausal brains. (A) The boxplot of the normalized data. Different colors represent different datasets. Rows represent samples, and columns represent the gene expression values in the samples. (B) PCA results after batch removal for multiple datasets. Different colors represent different datasets. As shown in the schematic diagram, five datasets are separated without any intersection. (C) Differential analysis of expressed genes in the brains between postmenopausal mice and postmenopausal women. (D) The expression distribution of ACTA2, CAMK2D, FNBP1L, ARL1, HEBP1, CAST, CLIC1, LPCAT4, MAST3, and DOCK9 in the brains of pre (age: 20–50) and postmenopausal (age > 50) women. **p < 0.01, and ***p < 0.001, asterisks (*) stand for significance levels. The statistical difference between the two groups was compared through the T test.

Article Snippet: Primary antibodies, rabbit anti JNK (pT183/pY185) (4668, cell signaling), and rabbit anti LPCAT4 (17905- 1- AP, Proteintech) were used to analyze the protein expression levels.

Techniques: Gene Expression, Expressing

Journal: The FASEB Journal

Article Title: Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas’ disease progression

doi: 10.1096/fj.202200427rr

Figure Lengend Snippet: FIGURE 9 Gene expression study of blood monocytes in pre- and postmenopausal females. (A–D) Upregulated genes (ARL1, DCUN1D1, PPP2CA, SLC24A3) in blood monocytes. (E–H) Downregulated genes (KRT1, LPCAT4, PCSK1N, HEBP1) in blood monocytes. (I) Sensitivity and specificity comparison of different protein expressions in gliomas associated with glioma prognosis by the area under the ROC curve.

Article Snippet: Primary antibodies, rabbit anti JNK (pT183/pY185) (4668, cell signaling), and rabbit anti LPCAT4 (17905- 1- AP, Proteintech) were used to analyze the protein expression levels.

Techniques: Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Periodic heat waves-induced neuronal etiology in the elderly is mediated by gut-liver-brain axis: a transcriptome profiling approach

doi: 10.1038/s41598-024-60664-9

Figure Lengend Snippet: Heat stress augmented the secretion of hepatic ORM2 and its abundance in the hippocampus along with the frontal cortex, traversing through leaky Blood–Brain-Barrier (BBB). ( A ) ORM2 immunoreactivity was shown by immunohistochemistry in liver sections from Control and Heat Stress mouse groups; Images were taken at 20X magnification and displayed with a scale of 200 μm. ( B ) ORM2 immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. ( C ) IL-1β immunoreactivity was shown by immunohistochemistry in small intestinal sections from Control and Heat Stress mouse groups; Images were taken at 20X magnification and displayed with a scale of 100 μm. ( D ) IL-1β immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. ( E ) The bar graph was plotted as the difference in ratio value of the relative abundance of the gut microbial phyla Firmicutes and Bacteroidetes compared between control and heat stress groups. The p value was derived through an unpaired t-test, with the significance level set at p < 0.05. ( F ) A correlation analysis was conducted between the gut dysbiosis marker Firmicutes/Bacteroidetes ratio and the abundance of ORM2 in liver tissues. The correlation plot includes Pearson's linear regression depicted in red, along with a 95% confidence interval. ( G ) Claudin5 (red) and CD31 (green) were dual labeled through immunofluorescence staining with colocalization (yellow) observed at 60X (oil) magnification. The images were presented with a scale of 20 μm in the brain sections from both the Control and Heat Stress mouse groups. ( H ) Claudin5-CD31 colocalization (yellow) events in both groups were quantified as arbitrary fluorescent units from six distinct microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. ( I ) ORM2 immunoreactivity was shown by immunohistochemistry in brain sections (hippocampus and frontal cortex) from Control and Heat Stress mouse groups; Images were taken at 10X, 40X magnification displayed with the scale of 200 μm. ( J ) ORM2 immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05.

Article Snippet: The primary antibody against Orosomucoid 2 (ORM2) was purchased from Proteintech Group Inc (Rosemont, IL, USA).

Techniques: Immunohistochemistry, Control, Derivative Assay, Marker, Labeling, Immunofluorescence, Staining

Journal: eLife

Article Title: Single-cell profiling reveals the intratumor heterogeneity and immunosuppressive microenvironment in cervical adenocarcinoma

doi: 10.7554/eLife.97335

Figure Lengend Snippet:

Article Snippet: Antibody , ORM1/2 rabbit mAb , ABclonal , Cat# A19736 , .

Techniques: Recombinant

Journal: Pharmacological research

Article Title: Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD.

doi: 10.1016/j.phrs.2025.107728

Figure Lengend Snippet: Fig. 5. Liver-specifically restoring miR-122 inhibited lipid peroxidation and liver injury induced by iron overload and miR-122 knockout. (A) miR-122 expression and (B) iron content in the liver of miR-122+/+ and miR-122-/- mice with iron overload (IO) and tail-vein injection of recombinant rAAV8 carrying miR-122 (AAV8). (C) Liver fat content and (D) PUFAs proportion of miR-122+/+ and miR-122-/- mice with iron overload and AAV8 treatment. (E) Liver MDA content and (F) liver LPO content of miR-122+/+ and miR-122-/- mice with iron overload and AAV8 treatment. (G) Serum ALT content and (H) serum AST content of miR-122+/+ and miR- 122-/- mice with iron overload and AAV8 treatment. (I) Prussian blue staining, H&E staining, oil red staining, Sirius red staining, and α-SMA staining of liver slices. Scale bar: 100 μm. (J-Q) mRNA expressions of Aacs, Fads2, Agpat1, Mogat2, Slc27a1, Cyp2b9, Cyp2a22, and Cyp3a11 in the liver of miR-122+/+ and miR-122-/- mice with iron overload (IO) and tail-vein injection of recombinant rAAV8 carrying miR-122 (AAV8). (R) Protein expressions of Aacs, Fads2, Agpat1, Mogat2, and Slc27a1 in the liver of miR-122+/+ and miR-122-/- mice with iron overload and AAV8 treatment. * : p < 0.05; **: p < 0.01; *** : p < 0.001, tested by one-way ANOVA with post-hoc Tukey’s multiple comparisons, sample size was presented in figure.

Article Snippet: Antibodies used in this study were β-actin (1:5000, Cat. 20536–1-AP, Proteintech, China), Aacs (1:500, Cat. 13815–1-AP, Proteintech, China), Fads2 (1:500, Cat. 28034–1-AP, Proteintech, China), Agpat1 (1:500, Cat. 10601–1-AP, Proteintech, China), Mogat2 (1:500, Cat. 19514–1-AP, Proteintech, China), Slc27a1 (1:500, Cat. DF7716, Affinity Biosciences, China).

Techniques: Knock-Out, Expressing, Injection, Recombinant, Staining

Journal: Pharmacological research

Article Title: Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD.

doi: 10.1016/j.phrs.2025.107728

Figure Lengend Snippet: Fig. 6. Systematically restoring miR-122 inhibited lipid peroxidation and liver injury induced by iron overload and miR-122 knockout. (A) miR-122 expression and (B) iron content in the liver with miR-122 knockout (KO), iron overload (IO), and tail-vein injection of miR-122 agomir. (C) Fat content and (D) PUFAs proportion of the liver with miR-122 KO, IO, and miR-122 agomir treatments. (E) MDA content and (F) LPO content of the liver with miR-122 KO, IO, and miR-122 agomir treatments. (G) Serum ALT content and (H) serum AST content of mice with miR-122 KO, IO, and miR-122 agomir treatments (I) Prussian blue staining, H&E staining, oil red staining, Sirius red staining, and α-SMA staining of liver slices. Scale bar: 100 μm. (J) mRNA expressions of Aacs, Fads2, Agpat1, Mogat2, Slc27a1, Cyp2b9, Cyp2a22, and Cyp3a11 in the liver with miR-122 knockout (KO), iron overload (IO), and tail-vein injection of miR-122 agomir. (K) Protein expressions of Aacs, Fads2, Agpat1, Mogat2, and Slc27a1 in the liver with miR-122 KO, IO, and miR-122 agomir treatments. * : p < 0.05; **: p < 0.01; *** : p < 0.001, tested by one-way ANOVA with post-hoc Tukey’s multiple comparisons, sample size was presented in figure.

Article Snippet: Antibodies used in this study were β-actin (1:5000, Cat. 20536–1-AP, Proteintech, China), Aacs (1:500, Cat. 13815–1-AP, Proteintech, China), Fads2 (1:500, Cat. 28034–1-AP, Proteintech, China), Agpat1 (1:500, Cat. 10601–1-AP, Proteintech, China), Mogat2 (1:500, Cat. 19514–1-AP, Proteintech, China), Slc27a1 (1:500, Cat. DF7716, Affinity Biosciences, China).

Techniques: Knock-Out, Expressing, Injection, Staining

Journal: Pharmacological research

Article Title: Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD.

doi: 10.1016/j.phrs.2025.107728

Figure Lengend Snippet: Fig. 7. A high-throughput screening identified dihydroberberine to upregulate miR-122 expression and prevent iron overload-induced MASLD. (A) The high- throughput screening results. Luminescence of the luciferase reporter were detected to represent miR-122 expression. The relative luminescence intensity was calculated by luminescence intensity of compound / luminescence intensity of blank control. Data were from two biological replicates. (B) The scheme of dihy droberberine (DHB) intervention in high-iron diet induced MASLD model. Dihydroberberine was i.p. administrated at 10 mg/kg, twice per week for 4 weeks. (C) The liver iron content. (D) The liver miR-122 expression level. (E) The liver TG content and (F) the liver total lipid content. (G) Oil red staining, Sirius red staining, and α-SMA staining of liver slices. Scale bar: 50 μm. (H) The liver weight / body weight ratio of mice. (I) Hepatic MDA content and (J) hepatic LPO content. (K) Total PUFAs proportion and two specific PUFA proportion, (L) C20:3n3 and (M) C22:6n3, in the liver. (N) mRNA expressions of Aacs, Fads2, Agpat1, Mogat2, Slc27a1, Cyp2a22, and Cyp3a11 in the liver. (O) Protein expressions of Aacs, Fads2, Agpat1, Mogat2, and Slc27a1 in the liver. * : p < 0.05; * *: p < 0.01; * ** : p < 0.001, tested by one-way ANOVA with post-hoc Tukey’s multiple comparisons, n = 6.

Article Snippet: Antibodies used in this study were β-actin (1:5000, Cat. 20536–1-AP, Proteintech, China), Aacs (1:500, Cat. 13815–1-AP, Proteintech, China), Fads2 (1:500, Cat. 28034–1-AP, Proteintech, China), Agpat1 (1:500, Cat. 10601–1-AP, Proteintech, China), Mogat2 (1:500, Cat. 19514–1-AP, Proteintech, China), Slc27a1 (1:500, Cat. DF7716, Affinity Biosciences, China).

Techniques: High Throughput Screening Assay, Expressing, Luciferase, Control, Staining

Journal: Molecular Biology of the Cell

Article Title: Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling

doi: 10.1091/mbc.E20-05-0303

Figure Lengend Snippet: Genetic analyses of Shs1 and Cdc12 AH domains. (A) Viability of cdc12-6 cells expressing indicated SHS1 alleles expressed from the endogenous locus (with 3xHA tag, unless indicated with GFP tag, which lacks 3xHA) based on tetrad dissections at permissive temperature (24°C). Cells from genotypes labeled in blue appeared normal, without obvious septin defects. Genotypes in orange were sick, with partially or fully penetrant septin defects. Genotypes labeled in red were inviable. (B) DIC images of cdc12-6 shs1 mutants (see A) at 24°C. Scale bar, 5 μm. (C) Heterozygous diploids expressing the indicated Shs1 protein fused to GFP from the SHS1 locus. Scale bar, 5 μm. (D) Scatter plot quantifying cdc12-6-SpoVM septin complex adsorption onto different membrane curvatures. Black bars represent the mean. Error bars are the SD for more than 30 measured beads at each curvature across three replicates. Adsorption of cdc12-6-SpoVM complexes was significantly greater on a membrane curvature of 2 μm −1 than on curvatures of 0.67 and 0.4 μm −1 ; ** ( p < 0.01 and p < 0.0001, respectively). ns, adsorption was not significantly different. (E) Western blot comparing expression of indicated Cdc12 chimeras fused to 3xHA epitope in heterozygous diploids.

Article Snippet: A polyclonal rabbit α-HA epitope antibody preparation was used at 1:2000 dilution (Rockland Immunochemicals).

Techniques: Expressing, Labeling, Adsorption, Membrane, Western Blot

Journal: Frontiers in Microbiology

Article Title: Identification of a Novel Serum Biomarker for Tuberculosis Infection in Chinese HIV Patients by iTRAQ-Based Quantitative Proteomics

doi: 10.3389/fmicb.2018.00330

Figure Lengend Snippet: Differentially expressed proteins in the sera of HIV positive patients with active tuberculosis (TB) relative to controls (only HIV positive patients).

Article Snippet: The concentrations of ENG (CUSABIO, CSB-E10030h, China), PSMB2 (CUSABIO, CSB-E17836h, China), HSP90AA1 (CUSABIO, CSB-E13462h, China), HSPA8 (CUSABIO, CSB-EL010829HU, China), CHI3L1 (CUSABIO, CSB-E13608h, China), CAP1 (CUSABIO, CSB-EL004486HU, China), ORM1 (CUSABIO, CSB-EL017237HU, China), RETN (CUSABIO, CSB-E06884h, China), LPA (CUSABIO, CSB-EQ028005HU, China) and ZYX (CUSABIO, CSB-EL027165HU, China) in the serum samples were quantified using ELISA according to the manufacturer’s instructions.

Techniques: