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A–C Bright-field, fluorescence, and scanning electron microscope images of SP, scale bar=50 μm; D Schematic illustration of AD-MSC-exo@SP_gel preparation (Created by BioRender); E Evaluation of Hydrogel adhesion after the addition of different concentrations of genipin; F Observation of Hydrogel morphology by scanning electron microscopy; G Degradation rate of Hydrogel; H Release profile of <t>exosomes;</t> I : Absorption peak (~680 nm) and corresponding fluorescence characteristics of SP; J: Assessment of the biocompatibility of AD-MSC-exo@SP_gel by incubating with HDF, HaCaT, and HUVEC cells related to skin tissue, scale bar=50 μm; K Hemocompatibility test of AD-MSC-exo@SP_gel. * indicates a statistically significant difference ( P < 0.05) between the two groups, with all cell experiments repeated three times.
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A–C Bright-field, fluorescence, and scanning electron microscope images of SP, scale bar=50 μm; D Schematic illustration of AD-MSC-exo@SP_gel preparation (Created by BioRender); E Evaluation of Hydrogel adhesion after the addition of different concentrations of genipin; F Observation of Hydrogel morphology by scanning electron microscopy; G Degradation rate of Hydrogel; H Release profile of <t>exosomes;</t> I : Absorption peak (~680 nm) and corresponding fluorescence characteristics of SP; J: Assessment of the biocompatibility of AD-MSC-exo@SP_gel by incubating with HDF, HaCaT, and HUVEC cells related to skin tissue, scale bar=50 μm; K Hemocompatibility test of AD-MSC-exo@SP_gel. * indicates a statistically significant difference ( P < 0.05) between the two groups, with all cell experiments repeated three times.
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A–C Bright-field, fluorescence, and scanning electron microscope images of SP, scale bar=50 μm; D Schematic illustration of AD-MSC-exo@SP_gel preparation (Created by BioRender); E Evaluation of Hydrogel adhesion after the addition of different concentrations of genipin; F Observation of Hydrogel morphology by scanning electron microscopy; G Degradation rate of Hydrogel; H Release profile of exosomes; I : Absorption peak (~680 nm) and corresponding fluorescence characteristics of SP; J: Assessment of the biocompatibility of AD-MSC-exo@SP_gel by incubating with HDF, HaCaT, and HUVEC cells related to skin tissue, scale bar=50 μm; K Hemocompatibility test of AD-MSC-exo@SP_gel. * indicates a statistically significant difference ( P < 0.05) between the two groups, with all cell experiments repeated three times.

Journal: NPJ Regenerative Medicine

Article Title: Novel approach for diabetic wound healing: adipose-derived mesenchymal stromal cells Exo@SPHydrogel combined with laser therapy

doi: 10.1038/s41536-026-00459-w

Figure Lengend Snippet: A–C Bright-field, fluorescence, and scanning electron microscope images of SP, scale bar=50 μm; D Schematic illustration of AD-MSC-exo@SP_gel preparation (Created by BioRender); E Evaluation of Hydrogel adhesion after the addition of different concentrations of genipin; F Observation of Hydrogel morphology by scanning electron microscopy; G Degradation rate of Hydrogel; H Release profile of exosomes; I : Absorption peak (~680 nm) and corresponding fluorescence characteristics of SP; J: Assessment of the biocompatibility of AD-MSC-exo@SP_gel by incubating with HDF, HaCaT, and HUVEC cells related to skin tissue, scale bar=50 μm; K Hemocompatibility test of AD-MSC-exo@SP_gel. * indicates a statistically significant difference ( P < 0.05) between the two groups, with all cell experiments repeated three times.

Article Snippet: The results showed (Figs. ) that the diameter of AD-MSC-derived exosomes ranged from approximately 50 to 150 nm, with high expression of exosome surface markers CD9 and CD63, while the negative control gene Calnexin showed no expression, indicating successful isolation of AD-MSC-derived exosomes.

Techniques: Fluorescence, Microscopy, Electron Microscopy