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A light micrograph of the <t>ACTIVA</t> <t>BioACTIVE</t> group. A1-A3; after 15 days post-treatment interval, (A1) The pulp tissue showed pulp fibrosis (arrows) and loss of odontoblastic layer (arrowheads), (A2) Immunohistochemical localization of fibronectin (arrows) was slightly detected in the pulp fibrosis and strongly noticed in the dentin layer at the pulp periphery, (A3) Immunohistochemical localization of OPN was nearly negative expressed on the pulp and dentin. (B1-B2; after 30 days post-treatment interval, (B1) The pulp tissue showed diffuse pulp fibrosis, nearly occupying the pulp space (arrows), and loss of odontoblastic layer (arrowheads), (B2) Immunohistochemical localization of fibronectin was strongly detected in the pulp matrix and the areas of pulp fibrosis (arrows), as well as in a small, calcified pulp stone (curved arrow), (B3) Immunohistochemical localization of OPN was strongly detected in the pulp matrix and the areas of pulp fibrosis (arrows) as well as in multiple calcified pulp stones (curved arrows). (D) Dentin, (P) pulp. (Orig. Mag.X400)
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A light micrograph of the ACTIVA BioACTIVE group. A1-A3; after 15 days post-treatment interval, (A1) The pulp tissue showed pulp fibrosis (arrows) and loss of odontoblastic layer (arrowheads), (A2) Immunohistochemical localization of fibronectin (arrows) was slightly detected in the pulp fibrosis and strongly noticed in the dentin layer at the pulp periphery, (A3) Immunohistochemical localization of OPN was nearly negative expressed on the pulp and dentin. (B1-B2; after 30 days post-treatment interval, (B1) The pulp tissue showed diffuse pulp fibrosis, nearly occupying the pulp space (arrows), and loss of odontoblastic layer (arrowheads), (B2) Immunohistochemical localization of fibronectin was strongly detected in the pulp matrix and the areas of pulp fibrosis (arrows), as well as in a small, calcified pulp stone (curved arrow), (B3) Immunohistochemical localization of OPN was strongly detected in the pulp matrix and the areas of pulp fibrosis (arrows) as well as in multiple calcified pulp stones (curved arrows). (D) Dentin, (P) pulp. (Orig. Mag.X400)

Journal: BMC Oral Health

Article Title: Histopathological and immunohistochemical characterization of pulp tissue reaction to ACTIVA BioACTIVE base/liner in primary teeth pulpotomy: a randomized clinical trial

doi: 10.1186/s12903-025-06177-x

Figure Lengend Snippet: A light micrograph of the ACTIVA BioACTIVE group. A1-A3; after 15 days post-treatment interval, (A1) The pulp tissue showed pulp fibrosis (arrows) and loss of odontoblastic layer (arrowheads), (A2) Immunohistochemical localization of fibronectin (arrows) was slightly detected in the pulp fibrosis and strongly noticed in the dentin layer at the pulp periphery, (A3) Immunohistochemical localization of OPN was nearly negative expressed on the pulp and dentin. (B1-B2; after 30 days post-treatment interval, (B1) The pulp tissue showed diffuse pulp fibrosis, nearly occupying the pulp space (arrows), and loss of odontoblastic layer (arrowheads), (B2) Immunohistochemical localization of fibronectin was strongly detected in the pulp matrix and the areas of pulp fibrosis (arrows), as well as in a small, calcified pulp stone (curved arrow), (B3) Immunohistochemical localization of OPN was strongly detected in the pulp matrix and the areas of pulp fibrosis (arrows) as well as in multiple calcified pulp stones (curved arrows). (D) Dentin, (P) pulp. (Orig. Mag.X400)

Article Snippet: Eighty primary first molars were allocated into two groups (40 molars/group) randomly according to different pulpotomy agents: Group I (experimental group) ( n = 40): primary first molars were treated with ACTIVA BioACTIVE Base/Liner (Pulpdent, Watertown, MA, USA).

Techniques: Immunohistochemical staining