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Table 3

Journal: Nature protocols

Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

doi: 10.1038/nprot.2017.016

Figure Lengend Snippet: Table 3

Article Snippet: PROCEDURE Designing a custom sgRNA library o TIMING 3–5 w; 1 w hands-on list-behavior=simple prefix-word= mark-type=none max-label-size=1 1 Construct a pooled sgRNA library by designing and cloning a custom sgRNA library (Steps 1–17) or amplifying a ready-made library from Addgene (Skip to Step 18).

Techniques: Sequencing