Review




Structured Review

Bachem substrate ac-devd-afc #4027914
Substrate Ac Devd Afc #4027914, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/substrate+ac+devd+afc++4027914/pm40331965-295-1-6
Average 90 stars, based on 1 article reviews
substrate ac-devd-afc #4027914 - by Bioz Stars, 2026-11
90/100 stars

Images

Related Articles

Activity Assay:


Article Title: Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation.
Article Snippet: Accepted Manuscript Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation Anja Pišlar, Biljana Božić, Nace Zidar, Janko Kos PII: S0028-3908(16)30523-8 DOI: 10.1016/j.neuropharm.2016.11.019 Reference: NP 6515 To appear in: Neuropharmacology Received Date: 28 June 2016 Revised Date: 8 October 2016 Accepted Date: 22 November 2016 Please cite this article as: Pišlar, A., Božić, B., Zidar, N., Kos, J., Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation, Neuropharmacology (2016), doi: 10.1016/ j.neuropharm.2016.11.019.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Intracellular cathepsin C levels determine sensitivity of cells to leucyl-leucine methyl ester-triggered apoptosis.
Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.15326 This article is protected by copyright.. All rights reserved PROF. BORIS TURK (Orcid ID : 0000-0002-9007-5764) Received Date : 04-Jul-2019 Revised Date : 06-Feb-2020 Accepted Date : 01-Apr-2020 Color : Fig 1-9

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel Inhibition.
Article Snippet: Active caspases -3, -8, and -9 predominantly cleaved fluorogenic substrates (Ac-DEVD-AMC, Ac-IETD-AFC, and Ac-LEHD-AFC) (Bachem AG., Bubendorf, Switzerland) to the AMC and AFCs following incubation with apoptotic cell lysates in black plates, respectively.

FACS:


Article Title: Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation.
Article Snippet: Accepted Manuscript Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation Anja Pišlar, Biljana Božić, Nace Zidar, Janko Kos PII: S0028-3908(16)30523-8 DOI: 10.1016/j.neuropharm.2016.11.019 Reference: NP 6515 To appear in: Neuropharmacology Received Date: 28 June 2016 Revised Date: 8 October 2016 Accepted Date: 22 November 2016 Please cite this article as: Pišlar, A., Božić, B., Zidar, N., Kos, J., Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation, Neuropharmacology (2016), doi: 10.1016/ j.neuropharm.2016.11.019.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Intracellular cathepsin C levels determine sensitivity of cells to leucyl-leucine methyl ester-triggered apoptosis.
Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.15326 This article is protected by copyright.. All rights reserved PROF. BORIS TURK (Orcid ID : 0000-0002-9007-5764) Received Date : 04-Jul-2019 Revised Date : 06-Feb-2020 Accepted Date : 01-Apr-2020 Color : Fig 1-9

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel Inhibition.
Article Snippet: Active caspases -3, -8, and -9 predominantly cleaved fluorogenic substrates (Ac-DEVD-AMC, Ac-IETD-AFC, and Ac-LEHD-AFC) (Bachem AG., Bubendorf, Switzerland) to the AMC and AFCs following incubation with apoptotic cell lysates in black plates, respectively.



Similar Products

94
MedChemExpress ac devd afc
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Ac Devd Afc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/Ac-DEVD-AFC/pmc12997193-46-32-44
Average 94 stars, based on 1 article reviews
ac devd afc - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

94
MedChemExpress protease inhibitors
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Protease Inhibitors, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/Ac-DEVD-AFC/pmc13097790-582-13-15
Average 94 stars, based on 1 article reviews
protease inhibitors - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

94
MedChemExpress casp substrates ac devd afc
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Casp Substrates Ac Devd Afc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/Ac-DEVD-AFC/10__1016_slash_j__aquaculture__2025__742828-123-1-10
Average 94 stars, based on 1 article reviews
casp substrates ac devd afc - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

90
AAT Bioquest ac-devd-afc
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Ac Devd Afc, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/z+ietd+afc/pm40170111-107-9-11
Average 90 stars, based on 1 article reviews
ac-devd-afc - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Bachem substrate ac-devd-afc #4027914
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Substrate Ac Devd Afc #4027914, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/substrate+ac+devd+afc++4027914/pm40331965-295-1-6
Average 90 stars, based on 1 article reviews
substrate ac-devd-afc #4027914 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

86
Macklin Inc ac devd afc
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Ac Devd Afc, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac+devd+afc/ac+cho+devd/10__1016_slash_j__cpb__2025__100487-219-30-31
Average 86 stars, based on 1 article reviews
ac devd afc - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

Image Search Results


Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Journal: Neoplasia (New York, N.Y.)

Article Title: Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors

doi: 10.1016/j.neo.2026.101295

Figure Lengend Snippet: Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Article Snippet: The payloads used for antibodies conjugation, MC-GGFG-DXD (DXd, catalog reference: HY-13631E), MC-Val-Cit-PAB-MMAF (Vc-MMAF, catalog reference: HY-112786) and McMMAF (catalog reference: HY-15578), the belantamab mafodotin ADC (catalog reference: HY-P3239), as well as the Ac-DEVD-AFC (catalog reference: HY-P1005) substrate for caspase-3 activity detection, were purchased from MedChem Express (Princeton, NJ, USA).

Techniques: Flow Cytometry, Apoptosis Assay, Western Blot, Control, Molecular Weight, Activity Assay, Fluorescence