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<t>ELISA</t> cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
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<t>ELISA</t> cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
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ELISA cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

Journal: Cell Reports

Article Title: Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants

doi: 10.1016/j.celrep.2023.112503

Figure Lengend Snippet: ELISA cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also Figure S1 .

Article Snippet: ABTS Chromogen/substrate solution for ELISA , Thermo Fisher Scientific , Cat#00-2024.

Techniques: Enzyme-linked Immunosorbent Assay, Bioprocessing, Isolation, Activity Assay, Binding Assay

Journal: Cell Reports

Article Title: Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants

doi: 10.1016/j.celrep.2023.112503

Figure Lengend Snippet:

Article Snippet: ABTS Chromogen/substrate solution for ELISA , Thermo Fisher Scientific , Cat#00-2024.

Techniques: Virus, Recombinant, FLAG-tag, Variant Assay, Marker, Staining, Enzyme-linked Immunosorbent Assay, Luciferase, Cell Culture, Lysis, Plasmid Preparation, Transgenic Assay, Software, Sterility, Suction Filtration