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The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + <t>ABA</t> + NaCl); ( C ) circular dichroism spectra of <t>aptamer</t> before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.
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The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + <t>ABA</t> + NaCl); ( C ) circular dichroism spectra of <t>aptamer</t> before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.
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The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + <t>ABA</t> + NaCl); ( C ) circular dichroism spectra of <t>aptamer</t> before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.
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The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + ABA + NaCl); ( C ) circular dichroism spectra of aptamer before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.

Journal: Biosensors

Article Title: A SERS/LSPR Dual-Signal Aptamer Sensor for Abscisic Acid Detection Based on Unmodified Gold Nanoparticles

doi: 10.3390/bios16030152

Figure Lengend Snippet: The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + ABA + NaCl); ( C ) circular dichroism spectra of aptamer before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.

Article Snippet: The ABA aptamer sequence was ATG GGT TAG GTG GAG GTG ATT CCG GGA ATT CGC CCT AAA TAC GAG CAA C oligonucleotide chain composed of 52 bases [ ], which was purchased from Sangon Biotech Co., Ltd., (Shanghai, China).

Techniques: Circular Dichroism, Binding Assay

The SERS intensity ( A ) and A620/A528 ( B ) of dual-signal output aptamer sensor for blank, ABA, GA3, VC, Ethylene, and β-Car. The concentration of ABA was 2 µM, while other interfering molecules were 10 µM. Error bars show the standard deviation of three repeated experiments.

Journal: Biosensors

Article Title: A SERS/LSPR Dual-Signal Aptamer Sensor for Abscisic Acid Detection Based on Unmodified Gold Nanoparticles

doi: 10.3390/bios16030152

Figure Lengend Snippet: The SERS intensity ( A ) and A620/A528 ( B ) of dual-signal output aptamer sensor for blank, ABA, GA3, VC, Ethylene, and β-Car. The concentration of ABA was 2 µM, while other interfering molecules were 10 µM. Error bars show the standard deviation of three repeated experiments.

Article Snippet: The ABA aptamer sequence was ATG GGT TAG GTG GAG GTG ATT CCG GGA ATT CGC CCT AAA TAC GAG CAA C oligonucleotide chain composed of 52 bases [ ], which was purchased from Sangon Biotech Co., Ltd., (Shanghai, China).

Techniques: Concentration Assay, Standard Deviation