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histone 3 abcam ab18521 1 5000  (Danaher Inc)


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    Danaher Inc histone 3 abcam ab18521 1 5000
    Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. <t>Histone</t> <t>3</t> was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.
    Histone 3 Abcam Ab18521 1 5000, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab18521/pmc11344828-206-14-16
    Average 86 stars, based on 1 article reviews
    histone 3 abcam ab18521 1 5000 - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Premature aging effects on COVID-19 pathogenesis: new insights from mouse models"

    Article Title: Premature aging effects on COVID-19 pathogenesis: new insights from mouse models

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-70612-2

    Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. Histone 3 was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.
    Figure Legend Snippet: Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. Histone 3 was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.

    Techniques Used: Western Blot, Control, Mutagenesis, Derivative Assay

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    Recombinant:

    Article Title: Translation stress and collided ribosomes are co-activators of cGAS
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Vinculin Sigma Cat# V9131; RRID: AB_477629 Histone H3 Abcam Cat# ab18521; RRID: AB_732917 hcGAS Cell signaling Technology Cat# 15102; RRID: AB_2732795 STING Cell signaling Technology Cat# 13647; RRID: AB_2732796 IRF3 Cell signaling Technology Cat# 4302; RRID: AB_1904036 P-IRF3 Cell signaling Technology Cat# 4947; RRID: AB_823547 TBK1 Cell signaling Technology Cat# 3504; RRID: AB_2255663 P-TBK1 Cell signaling Technology Cat# 5483; RRID: AB_10693472 ASCC3 Dango et al., 2011 N/A ASCC2 Abcam Cat# Ab168811; RRID: AB_2832200 ASCC1 Proteintech Cat# 12301-1-AP; RRID: AB_2059350 TRIP4 NOVUS Cat# NB100-419; RRID: AB_10000684 uL2 Abcam Cat# Ab169538; RRID: AB_2714187 eS24 Abcam Cat# Ab196652; RRID: AB_2714188 uS3 Bethyl Cat# A303-840A; RRID: AB_2620191 uS5 Bethyl Cat# A303-794A; RRID: AB_11218192 eS10 Abcam Cat# Ab151550; RRID: AB_2714147 eS8 Abcam Cat# Ab201454 RRID: AB_2833046 GFP Cell signaling Technology Cat# 2956; RRID:AB_1196615 MAVS Cell signaling Technology Cat# 3993; RRID: AB_1196615 P-STAT1 Cell signaling Technology Cat# 9167; RRID: AB_561284 STAT1 Cell signaling Technology Cat# #9172; RRID:AB_2198300 RSAD2 Cell signaling Technology Cat#13996; RRID:AB_2734772 IFIT2 Proteintech Cat# 12604-1-AP; RRID: AB_2864734 Flag Sigma Cat# F1804; RRID: AB_262044 RACK1 Abcam Cat# ab62735; RRID: AB_956255 ZNF598 GeneTex Cat# GTX119245; RRID: AB_10619017 gH2AX Sigma Cat# 05-636; RRID: AB_309864 dsDNA Abcam Cat# ab27156; RRID: AB_470907 Goat anti-Rabbit Alexa Fluor 594 ThermoFisher Scientific Cat# A-11012; RRID: AB_2534079 Goat anti-mouse Alexa Fluor 594 ThermoFisher Scientific Cat# A-11005; RRID: AB_2534073 Bacterial and virus strains BL21 DE3 ThermoFisher Scientific C600003 NEB 5-alpha Competent E. coli New England Biolabs C2987H One Shot ccdB Survival 2 T1R Competent Cells ThermoFisher Scientific A10460 Chemicals, peptides, and recombinant proteins Doxycycline Clontech 8634-1 3xFLAG peptide Peptide Chemistry, The Francis Crick Institute N/A 4-thiouridine Glentham Life Sciences GN6085 4-thiouracil Sigma-Aldrich 440736 (Continued on next page) Molecular Cell 81, 2808–2822.e1–e10, July 1, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTSEA biotin-XX linker ((MTSEA Biotincapcap; 2-((6-((6-((biotinoyl)amino) hexanoyl)amino)hexanoyl) amino) ethylmethanethiosulfonate)) Biotium BT90066 Cycloheximide Sigma-Aldrich C4859-1ML Emetine dihydrochloride BioVision 1970-50 Anisomycin APExBIO B6674 PreScission Protease GenScript Z02799 cGAS recombinant protein This paper N/A cGAS-8his recombinant protein This paper N/A hPrimpol1-8his recombinant protein This paper N/A eRF1-AAQ (human) Juszkiewicz et al., 2018 N/A Critical commercial assays Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S RNeasy kit QIAGEN 74104 miRNeasy kit QIAGEN 217004 RNA minElute clean-up kit QIAGEN 74204 PureLink RNA Mini kit Thermo Fisher Scientific 12183020 20,30-Cyclic GAMP Direct EIA Kit 2Bscientific K067-H1 mMACS Streptavidin Kit Miltenyi 130-074-101 Taqman Reverse Transcriptase Reagents Thermo Fisher Scientific N8080234 SilverQuest Silver Staining Kit Thermo Fisher Scientific LC6070 Duolink In Situ Red Starter Kit Mouse/ Rabbit Sigma DUO92101 Experimental models: Cell lines HEK293 The Francis Crick Institute Cell Services N/A HEK293T The Francis Crick Institute Cell Services N/A U2OS The Francis Crick Institute Cell Services N/A MRC5VA The Francis Crick Institute Cell Services N/A Flp-In T-REx HEK293 ThermoFisher Scientific R78007 Flp-In T-REx U2OS Arquint and Nigg, 2014 N/A MRC5VA Parent This paper N/A MRC5VA KO-7 This paper N/A MRC5VA KO-12 This paper N/A Flp-In T-REx U2OS Parent This paper N/A Flp-In T-REx U2OS KO-16 This paper N/A Flp-In T-REx U2OS KO-23 This paper N/A Flp-In T-REx HEK293-KAAA0 This paper N/A Flp-In T-REx HEK293-KAAA20 This paper N/A Flp-In T-REx U2OS CGASKO16-GFPVector This paper N/A Flp-In T-REx U2OS CGASKO16-GFPcGAS-WT This paper N/A Flp-In T-REx U2OS CGASKO16-GFPcGAS-C396A This paper N/A Flp-In T-REx U2OS CGASKO16-GFPcGAS-ED > AA This paper N/A Flp-In T-REx U2OS CGASKO16-GFPcGAS-K411A This paper N/A (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 81, 2808–2822.e1–e10, July 1, 2021



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    Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. <t>Histone</t> <t>3</t> was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.
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    Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. Histone 3 was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.

    Journal: Scientific Reports

    Article Title: Premature aging effects on COVID-19 pathogenesis: new insights from mouse models

    doi: 10.1038/s41598-024-70612-2

    Figure Lengend Snippet: Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. Histone 3 was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.

    Article Snippet: The following primary antibodies were used: anti-LAMINA/C (CST, 4777S, 1:1000), anti-progerin (abcam, ab66587, 1:1000), Histone 3 (abcam, ab18521, 1:5000).

    Techniques: Western Blot, Control, Mutagenesis, Derivative Assay